Farnesoid X Receptor Activation Prevents the Development of Vascular Calcification in ApoE−/− Mice With Chronic Kidney Disease

• Published in: Circulation research 2010-06, Vol.106 (12), p.1807-1817
• Main Authors: Miyazaki-Anzai, Shinobu, Levi, Moshe, Kratzer, Adelheid, Ting, Tabitha C, Lewis, Linda B, Miyazaki, Makoto
• Format: Article
• Language: English
• Subjects: Animals; Aorta - cytology; Aorta - drug effects; Aorta - metabolism; Apolipoproteins E - genetics; Apolipoproteins E - physiology; Biological and medical sciences; Calcinosis - physiopathology; Calcinosis - prevention & control; Cattle; Cell Differentiation - drug effects; Cells, Cultured; Chenodeoxycholic Acid - analogs & derivatives; Chenodeoxycholic Acid - pharmacology; Chenodeoxycholic Acid - therapeutic use; Chronic Disease; Disease Models, Animal; Fundamental and applied biological sciences. Psychology; Kidney Diseases - physiopathology; Kidneys; Male; Medical sciences; Mice; Mice, Knockout; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Osteogenesis - drug effects; Receptors, Cytoplasmic and Nuclear - agonists; Receptors, Cytoplasmic and Nuclear - drug effects; Receptors, Cytoplasmic and Nuclear - physiology; Renal failure; Signal Transduction - physiology; Triglycerides - metabolism; Urinary system involvement in other diseases. Miscellaneous; Vascular Diseases - physiopathology; Vertebrates: cardiovascular system
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/CIRCRESAHA.109.212969

RATIONALE:Vascular calcification is highly associated with cardiovascular morbidity and mortality, especially in patients with chronic kidney disease. The nuclear receptor farnesoid X receptor (FXR) has been implicated in the control of lipid, carbohydrate and bile acid metabolism in several cell types. Although recent studies have shown that FXR is also expressed in vascular smooth muscle cells, its physiological role in vasculature tissue remains obscure. OBJECTIVE:Here, we have examined the role of FXR in vascular calcification. METHODS AND RESULTS:The FXR gene, a bile acid nuclear receptor, was highly induced during osteogenic differentiation of bovine calcifying vascular cells (CVCs) and in the aorta of apolipoprotein (Apo)E mice with chronic kidney disease which are common tissue culture and mouse model, respectively, for aortic calcification. FXR activation by a synthetic FXR agonist, 6α-ethyl chenodeoxycholic acid (INT-747) inhibited phosphate induced-mineralization and triglyceride accumulation in CVCs. FXR dominant negative expression augmented mineralization of CVCs and blocked the anticalcific effect of INT-747 whereas VP16FXR that is a constitutively active form reduced mineralization of CVCs. INT-747 treatment also increased phosphorylated c-Jun N-terminal kinase (JNK). SP600125 (specific JNK inhibitor) significantly induced mineralization of CVCs and alkaline phosphatase expression, suggesting that the anticalcific effect of INT-747 is attributable to JNK activation. We also found that INT-747 ameliorates chronic kidney disease induced-vascular calcification in 5/6 nephrectomized ApoE mice without affecting the development of atherosclerosis. CONCLUSIONS:These observations provide direct evidence that FXR is a key signaling component in regulation of vascular osteogenic differentiation and, thus representing a promising target for the treatment of vascular calcification.