Isolation and partial characterization of antibiotics produced by Paenibacillus elgii B69

• Published in: FEMS microbiology letters 2010-09, Vol.310 (1), p.32-38
• Main Authors: Wu, Xue-Chang, Shen, Xiao-Bo, Ding, Rui, Qian, Chao-Dong, Fang, Hai-Huan, Li, Ou
• Format: Article
• Language: English
• Subjects: Amino acids; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; antibiotic; Antibiotics; Antifungal activity; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Bacteria - drug effects; Bacteriology; biocontrol; Biological and medical sciences; Biological control; Cell culture; Chromatography, Gel; Chromatography, High Pressure Liquid; Cluster Analysis; Column chromatography; Culture Media - chemistry; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Drug Stability; Fundamental and applied biological sciences. Psychology; Fungi - drug effects; Fungicides; High performance liquid chromatography; Hot Temperature; Humans; Hydrogen-Ion Concentration; Liquid chromatography; Microbial Sensitivity Tests; Microbiology; Miscellaneous; Molecular Sequence Data; Molecular Weight; Organic chemistry; Paenibacillus; Paenibacillus - chemistry; Paenibacillus - classification; Paenibacillus - isolation & purification; Paenibacillus - metabolism; Paenibacillus elgii; Pelgipeptin; Pesticides; Phylogeny; Plant diseases; Polypeptin; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Stability analysis
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2010.02040.x

A newly isolated strain B69 with broad antimicrobial activity was identified as Paenibacillus elgii by 16S rRNA gene sequence analysis, along with physiological and biochemical characterization. Two antimicrobial compounds, named as Pelgipeptins A and B, were isolated from the culture medium using MCI GEL CHP20P column chromatography and HPLC methods. The molecular masses of Pelgipeptins A and B were 1072 and 1100 Da, respectively. The ESI-CID-MS and amino acid analysis suggested that both of them belonged to the polypeptin family, and Pelgipeptin A was unequivocally characterized as a new antibiotic. These two antibiotics were active against all the tested bacterial strains and displayed strong antifungal activity against several soil-borne fungal pathogens, with minimal inhibitory concentration values of 6.25-50 μg mL⁻¹. Furthermore, stability analysis indicated that the inhibitory activity of Pelgipeptins in the cell-free supernatant was unaffected during exposure to 60 °C for 2 h or a pH ranging from 1.0 to 8.0. Based on the strong antifungal activity and attractive biochemical properties, Pelgipeptins might provide an alternative resource of chemical pesticides for the biocontrol of plant diseases.