The effect of Saccharomyces boulardii on Candida albicans-infected human intestinal cell lines Caco-2 and Intestin 407

Abstract Saccharomyces boulardii is a probiotic strain that confers many benefits to human enterocolopathies and is used against a number of enteric pathogens. Candida albicans is an opportunistic pathogen that causes intestinal infections in immunocompromised patients, and after translocation into...

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Bibliographic Details
Published in:FEMS microbiology letters 2010-09, Vol.310 (1), p.17-23
Main Authors: Murzyn, Anna, Krasowska, Anna, Augustyniak, Daria, Majkowska-Skrobek, Grażyna, Łukaszewicz, Marcin, Dziadkowiec, Dorota
Format: Article
Language:English
Subjects:
Adhesion
Biological and medical sciences
Biotechnology
Caco‐2
Candida albicans
Candida albicans - growth & development
Candida albicans - pathogenicity
Candidiasis
Cell Adhesion
Cell culture
Cell Line
Cell lines
Cytokines
Epithelial cells
Epithelial Cells - microbiology
Fundamental and applied biological sciences. Psychology
Fungi
Gene expression
Gene Expression Profiling
Humans
IL-1β
Immunocompromised hosts
Inflammation
Interleukin 6
Interleukin 8
Interleukin-1beta - biosynthesis
Interleukin-6 - biosynthesis
Interleukin-8 - biosynthesis
Intestin 407
Intestine
Microbiology
Miscellaneous
Mushrooms
Mycology
Opportunist infection
Pathogens
Polymerase chain reaction
Probiotics
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces - growth & development
Saccharomyces - physiology
Saccharomyces boulardii
Translocation
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Summary:Abstract Saccharomyces boulardii is a probiotic strain that confers many benefits to human enterocolopathies and is used against a number of enteric pathogens. Candida albicans is an opportunistic pathogen that causes intestinal infections in immunocompromised patients, and after translocation into the bloodstream, is responsible for serious systemic candidiasis. In this study, we investigated the influence of S. boulardii cells and its culture extract on C. albicans adhesion to Caco-2 and Intestin 407 cell lines. We also tested the proinflammatory IL-1β, IL-6 and IL-8 cytokine expression by C. albicans-infected Caco-2 cells, using real-time RT-PCR. We found that both S. boulardii and its extract significantly inhibited C. albicans adhesion to epithelial cell lines. The IL-8 gene expression by C. albicans-infected Caco-2 cells was suppressed by the addition of S. boulardii extract. Our results indicate that S. boulardii affects C. albicans adhesion and reduces cytokine-mediated inflammatory host response.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2010.02037.x