Stabilization of amorphous calcium carbonate by phosphate rich organic matrix proteins and by single phosphoamino acids

Stable amorphous calcium carbonate (ACC) is a unique material produced naturally exclusively as a biomineral. It was demonstrated that proteins extracted from biogenic stable ACC induce and stabilize synthetic ACC in vitro. Polyphosphate molecules were similarly shown to induce amorphous calcium car...

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Bibliographic Details
Published in:Journal of structural biology 2010-08, Vol.171 (2), p.207-215
Main Authors: Bentov, Shmuel, Weil, Simy, Glazer, Lilah, Sagi, Amir, Berman, Amir
Format: Article
Language:English
Subjects:
Amorphous calcium carbonate (ACC)
Animals
Astacoidea - chemistry
Astacoidea - metabolism
Biomineralization
Calcium Carbonate - chemistry
Calcium Carbonate - metabolism
Cambaridae
Cherax quadricarinatus
Crayfish gastroliths
Crustacea
Male
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Phosphoamino acids
Phosphoamino Acids - chemistry
Phosphoamino Acids - metabolism
Phosphoproteins
Phosphoproteins - chemistry
Phosphoproteins - metabolism
Spectrum Analysis, Raman
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Summary:Stable amorphous calcium carbonate (ACC) is a unique material produced naturally exclusively as a biomineral. It was demonstrated that proteins extracted from biogenic stable ACC induce and stabilize synthetic ACC in vitro. Polyphosphate molecules were similarly shown to induce amorphous calcium carbonate formation in vitro. Accordingly, we tested the hypothesis that biogenic ACC induction and stabilization is mediated by the phosphorylated residues of phosphoproteins. We show that extracellular organic matrix extracted from gastroliths of the red claw crayfish Cherax quadricarinatus induce stable ACC formation in vitro. The proteinaceous fraction of this organic matrix is highly phosphorylated and is incorporated into the ACC mineral phase during precipitation. We have identified the major phosphoproteins of the organic matrix and showed that they have high calcium binding capacity. Based on the above, in vitro precipitation experiments with single phosphoamino acids were performed, indicating that phosphoserine or phosphothreonine alone can induce the formation of highly stable ACC. The results indicate that phosphoproteins may play a major role in the control of ACC formation and stabilization and that their phosphoamino acid moieties are key components in this process.
ISSN:1047-8477
1095-8657
DOI:10.1016/j.jsb.2010.04.007