Reduced plasma APOA1 level is associated with Gastric Tumor Growth in MKN45 mouse xenograft model

There is no suitable diagnostic and prognostic biomarker for gastric cancer. The biggest hurdles in biomarker discovery are (i) the low abundance of cancer cell-specific proteins that limits their detection and (ii) complex inter-patient variations that complicate the discovery process. To circumven...

Full description

Saved in:
Bibliographic Details
Published in:Journal of proteomics 2010-06, Vol.73 (8), p.1632-1640
Main Authors: Chong, Poh-Kuan, Lee, Huiyin, Zhou, Jianbiao, Liu, Shaw-Cheng, Loh, Marie Chiew Shia, So, Jimmy Bok Yan, Lim, Khong Hee, Yeoh, Khay-Guan, Lim, Yoon-Pin
Format: Article
Language:English
Subjects:
APOA1
Biological and medical sciences
Cancer marker
Diverse techniques
Fundamental and applied biological sciences. Psychology
Gastric cancer
Gastroenterology. Liver. Pancreas. Abdomen
Medical sciences
Molecular and cellular biology
Plasma
Proteomics
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:There is no suitable diagnostic and prognostic biomarker for gastric cancer. The biggest hurdles in biomarker discovery are (i) the low abundance of cancer cell-specific proteins that limits their detection and (ii) complex inter-patient variations that complicate the discovery process. To circumvent these issues, we conducted proteomics on the plasma of gastric cancer mouse xenograft and attempted to identify proteins released by cancer cells. MKN45 gastric cancer cells were subcutaneously implanted into immune-incompetent nude mice. Plasma samples collected from mice with different tumor sizes (low, mid and high tumor loads) were subjected to iTRAQ and mass spectrometric analyses. Detection of human APOA1 in mouse plasma was verified and its expression level was shown to be lower in mice with large tumors compared to those with small tumors. Studies on a panel of about 14 gastric cancer cell lines supported the notion that APOA1 in mouse plasma was of human gastric cancer cell origin. While the clinical utility of APOA1 remains to be ascertained with a larger scale study, the current work supported the feasibility of using mouse xenograft model for gastric cancer biomarker discovery. [Display omitted]
ISSN:1874-3919
DOI:10.1016/j.jprot.2010.04.005