The isolation and identification of predatory bacteria from a Microcystis algal bloom

Water samples were collected from a hypertrophic lake, Hartbeespoort dam, where lytic or predatory bacteria were isolated. The water samples were spread on BG 11 agar plates where upon incubation, plaques were observed on Microcystis lawns. Two isolates were further subjected to screening to evaluat...

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Bibliographic Details
Published in:African journal of biotechnology 2010-02, Vol.9 (5), p.663-671
Main Authors: Jabulani, R Gumbo, Gina, Ross, Thomas, E Cloete
Format: Article
Language:English
Subjects:
Agar
Algal blooms
Bacillus mycoides
Lakes
Microcystis
Plaques
Pseudomonas stutzeri
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Summary:Water samples were collected from a hypertrophic lake, Hartbeespoort dam, where lytic or predatory bacteria were isolated. The water samples were spread on BG 11 agar plates where upon incubation, plaques were observed on Microcystis lawns. Two isolates were further subjected to screening to evaluate their lytic activities on Microcystis cells and identification. Isolates B2 and B16 had a lytic effect on the Microcystis cells with isolate B16 having a greater effect than isolate B2. Isolate B2 was identified as Pseudomonas stutzeri with 99.9% certainty and B16 as Bacillus mycoides with 99.7% certainty using the API system.
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB09.834