In vitro antituberculosis activities of the constituents isolated from Haloxylon salicornicum

• Published in: Bioorganic & medicinal chemistry letters 2010-07, Vol.20 (14), p.4173-4176
• Main Authors: Bibi, Nazia, Tanoli, Sheraz Ahmad. K., Farheen, Sadia, Afza, Nighat, Siddiqi, Salman, Zhang, Ying, Kazmi, Shahana U., Malik, Abdul
• Format: Article
• Language: English
• Subjects: Alkaloids; Antitubercular Agents - isolation & purification; Antitubercular Agents - pharmacology; Antituberculosis activities; Biological and medical sciences; Chenopodiaceae - chemistry; Colorimetry; General pharmacology; Haloxylon salicornicum; In Vitro Techniques; Isoniazid; Medical sciences; Microbial Sensitivity Tests; Mycobacterium; Mycobacterium tuberculosis - drug effects; Mycobecterium tuberculosis; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/j.bmcl.2010.05.061

In vitro antituberculosis activities of series of four fractions and twenty (1–20) pure compounds included seven triterpenes, two alkaloids, two cycloheximide derivatives, two coumarins six sterol derivatives and a long chain alcohol, respectively, isolated from Haloxylon salicornicum were evaluated against Mycobecterium tuberculosis H37Rv. Actively growing cultures were tested by rapid colorimetric method while the stationary phase cultures were tested by drug exposure methods for bactericidal activity. The MIC values were found significant (50μg/ml) for the compounds 15, 19 and 20 where as rest of the compounds invariably showed MIC value of 100μg/ml against the logarithmic phase culture, these were compare to Isoniazid as control drug. In vitro antituberculosis activities of fractions and pure compounds (1–20) including seven triterpenes, two alkaloids, two cycloheximide derivatives, two coumarins six sterol derivatives and a long chain alcohol, respectively, isolated from Haloxylon salicornicum were determined against Mycobecterium tuberculosis H37Rv. Actively growing cultures were tested by rapid colorimetric method while the stationary phase cultures were tested by drug exposure methods for bactericidal activity. The MIC values were found to be 50μg/ml for compounds 15, 19 and 20 where as rest of the compounds invariably showed MIC value of 100μg/ml against the logarithmic phase culture. These were compare to Isoniazid as a control drug. The compounds exhibited no activity against the stationary phase culture of M. tuberculosis H37Rv up to 200μg/ml. Further studies are required to investigate the in vivo efficacies and activities of the compounds in combination with antimicrobials that are already being used for TB therapy.