Application of selected biological control agents in conjunction with tolclofos-methyl for the control of damping-off caused by Rhizoctonia solani

Selected Trichoderma and Bacillus isolates were tested as seed treatments with reduced concentrations of tolclofos-methyl against Rhizoctonia solani damping-off using cucumber as test plants. In vitro bioassays using filter paper disc infused with six concentrations [active ingredient (ai)] of tolcl...

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Bibliographic Details
Published in:African journal of biotechnology 2010-03, Vol.9 (12), p.1789-1796
Main Authors: K, S Yobo, M, D Laing, C, H Hunter
Format: Article
Language:English
Subjects:
Agar
Bacillus
Biological control
Cucumis sativus
Damping-off
Disease control
Filter paper
Greenhouses
Rhizoctonia solani
Seed treatments
Seeds
tolclofos-methyl
Trichoderma
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Summary:Selected Trichoderma and Bacillus isolates were tested as seed treatments with reduced concentrations of tolclofos-methyl against Rhizoctonia solani damping-off using cucumber as test plants. In vitro bioassays using filter paper disc infused with six concentrations [active ingredient (ai)] of tolclofos-methyl (0.005, 0.05, 0.125, 0.25, 0.5 and 1.5 g ai/l) showed that growth of Trichoderma isolates on agar plates were less inhibited at 0.005 and 0.05 g ai/l than 0.125 - 1.5 g ai/l tolclofos-methyl concentrations while Bacillus isolates were not affected by any of the six concentrations. In a greenhouse study with a 0.05 g ai/l tolclofos-methyl concentration, Trichoderma and Bacillus isolates applied alone or in combination to cucumber seeds resulted in a better disease control than the Trichoderma and Bacillus treatments used alone. As high as 86% disease control was achieved by combining 0.05 g ai/l tolclofos-methyl with 7. harzianum Isolate SYN which wassignificantly better (P = 0.001) than the 0.05 g ai/l tolclofos-methyl control. In all cases, additive effects of combining 0.05 g ai/l tolclofos-methyl with Trichoderma and Bacillus treatments were observed.
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB10.1171