Combining HPLC-GCXGC, GCXGC/ToF-MS, and Selected Ecotoxicity Assays for Detailed Monitoring of Petroleum Hydrocarbon Degradation in Soil and Leaching Water

HPLC-GCXGC/FID (high-performance liquid chromatography followed by comprehensive two-dimensional gas chromatography with flame-ionization detection) and GCXGC/ToF-MS (comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry) were used to study the biodegradation of petr...

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Bibliographic Details
Published in:Environmental science & technology 2009-10, Vol.43 (20), p.7651-7657
Main Authors: Mao, Debin, Lookman, Richard, Weghe, Hendrik Van De, Weltens, Reinhilde, Vanermen, Guido, Brucker, Nicole De, Diels, Ludo
Format: Article
Language:English
Subjects:
Aliivibrio fischeri
Applied sciences
Biodegradation
Biodegradation, Environmental
Characterization of Natural and Affected Environments
Chromatography
Chromatography, High Pressure Liquid
Exact sciences and technology
Fertilizers
Flame Ionization
Gas Chromatography-Mass Spectrometry
Hydrocarbons
Leaching
Lepidium sativum
Petroleum
Petroleum - analysis
Petroleum - toxicity
Pollution
Polycyclic Aromatic Hydrocarbons - analysis
Polycyclic Aromatic Hydrocarbons - metabolism
Soil - analysis
Soil contamination
Soil Microbiology
Soil Pollutants - analysis
Soil Pollutants - metabolism
Soils
Terpenes - analysis
Terpenes - metabolism
Toxicity
Toxicity Tests, Acute
Vibrio fischeri
Water - analysis
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Summary:HPLC-GCXGC/FID (high-performance liquid chromatography followed by comprehensive two-dimensional gas chromatography with flame-ionization detection) and GCXGC/ToF-MS (comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry) were used to study the biodegradation of petroleum hydrocarbons in soil microcosms during 20 weeks. Two soils were studied: one spiked with fresh diesel and one field sample containing weathered diesel-like oil. Nutrient amended and unamended samples were included. Total petroleum hydrocarbon (TPH) levels in spiked soil decreased from 15 000 to 7500 mg/kg d.m. and from 12 000 to 4000 mg/kg d.m. in the field soil. Linear alkanes and aromatic hydrocarbons were better biodegradable (>60% degraded) than iso-alkanes; cycloalkanes were least degradable (
ISSN:0013-936X
1520-5851
DOI:10.1021/es9015603