Evolving Trends in the Dereplication of Natural Product Extracts: New Methodology for Rapid, Small-Scale Investigation of Natural Product Extracts

The use of an HPLC bioactivity profiling/microtiter plate technique in conjunction with capillary probe NMR instrumentation and access to appropriate databases effectively short-circuits conventional dereplication procedures, necessarily based on multimilligram extracts, to a single, more rapid subm...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2008-09, Vol.71 (9), p.1595-1599
Main Authors: Lang, Gerhard, Mayhudin, Nor Ainy, Mitova, Maya I, Sun, Lin, van der Sar, Sonia, Blunt, John W, Cole, Anthony L. J, Ellis, Gill, Laatsch, Hartmut, Munro, Murray H. G
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biological Products - chemistry
chemical analysis
Chromatography, High Pressure Liquid
Combinatorial Chemistry Techniques - economics
Combinatorial Chemistry Techniques - methods
endophytes
Fundamental and applied biological sciences. Psychology
high performance liquid chromatography
Molecular Structure
nuclear magnetic resonance spectroscopy
Nuclear Magnetic Resonance, Biomolecular
Plant Extracts - chemistry
spectral analysis
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Summary:The use of an HPLC bioactivity profiling/microtiter plate technique in conjunction with capillary probe NMR instrumentation and access to appropriate databases effectively short-circuits conventional dereplication procedures, necessarily based on multimilligram extracts, to a single, more rapid submilligram operation. This approach to dereplication is illustrated using fungal or bacterial extracts that contain known compounds. In each case the dereplication steps were carried out on microgram quantities of extract and demonstrate the discriminating power of 1H NMR spectroscopy as a definitive dereplication tool.
ISSN:0163-3864
1520-6025
DOI:10.1021/np8002222