The Role of Surface Charging during the Coadsorption of Mercaptohexanol to DNA Layers on Gold:  Direct Observation of Desorption and Layer Reorientation

We study the coadsorption of mercaptohexanol onto preimmobilized oligonucleotide layers on gold. Monitoring the position of the DNA relative to the surface by optical means directly shows the mercaptohexanol-induced desorption of DNA and the reorientation of surface-tethered strands in situ and in r...

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Bibliographic Details
Published in:Langmuir 2006-06, Vol.22 (13), p.5560-5562
Main Authors: Arinaga, K., Rant, U., Tornow, M., Fujita, S., Abstreiter, G., Yokoyama, N.
Format: Article
Language:English
Subjects:
Adsorption
Base Sequence
Carbocyanines
Chemistry
Coated Materials, Biocompatible - chemistry
DNA - chemistry
Exact sciences and technology
Fluorescent Dyes
General and physical chemistry
Gold
Hexanols - chemistry
Materials Testing
Static Electricity
Sulfhydryl Compounds - chemistry
Surface Properties
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Summary:We study the coadsorption of mercaptohexanol onto preimmobilized oligonucleotide layers on gold. Monitoring the position of the DNA relative to the surface by optical means directly shows the mercaptohexanol-induced desorption of DNA and the reorientation of surface-tethered strands in situ and in real time. By simultaneously recording the electrochemical electrode potential, we are able to demonstrate that changes in the layer conformation are predominantly of electrostatic origin and can be reversed by applying external bias to the substrate.
ISSN:0743-7463
1520-5827
DOI:10.1021/la060324m