Interaction of DNA Oligomers with Cationic Lipidic Monolayers:  Complexation and Splitting

Interactions of native DNA with octadecylamine (ODA) and hexadecymdimethylammonium bromide (HTAB) monolayers at the air/water interface were studied by π−A isotherms, ellipsometry, and X-ray reflectivity. We show that the microscopic structure of ODA−DNA complexes is definitely consistent with a sin...

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Bibliographic Details
Published in:Langmuir 2007-04, Vol.23 (8), p.4414-4420
Main Authors: Erokhina, Svetlana, Berzina, Tatiana, Cristofolini, Luigi, Konovalov, Oleg, Erokhin, Victor, Fontana, Marco P
Format: Article
Language:English
Subjects:
Adsorption
Air
Amines - chemistry
Cations
Chemistry, Physical - methods
DNA - chemistry
Lipids - chemistry
Models, Molecular
Oligonucleotides - chemistry
Pressure
Quaternary Ammonium Compounds - chemistry
Surface Properties
Water - chemistry
X-Rays
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Summary:Interactions of native DNA with octadecylamine (ODA) and hexadecymdimethylammonium bromide (HTAB) monolayers at the air/water interface were studied by π−A isotherms, ellipsometry, and X-ray reflectivity. We show that the microscopic structure of ODA−DNA complexes is definitely consistent with a single-stranded form for DNA. On the contrary, with HTAB, DNA complexes in its native form. The crucial difference in the behavior of these two fairly similar lipids is due to the presence of the amine group in ODA. These results should be relevant to applications such as DNA chips and sensors.
ISSN:0743-7463
1520-5827
DOI:10.1021/la070069l