Modeling of RGDC Film Parameters Using X-ray Photoelectron Spectroscopy

Immobilization of peptides on surfaces is a common method to investigate biological response to biomaterials for the development of improved tissue engineering constructs. Peptide immobilization can be achieved by either physical adsorption or covalent attachment on the surface. In this work, the RG...

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Bibliographic Details
Published in:Langmuir 2005-08, Vol.21 (16), p.7061-7065
Main Authors: Popat, Ketul C, Leary Swan, Erin E, Desai, Tejal A
Format: Article
Language:English
Subjects:
Adsorption
Aluminum - chemistry
Animals
Bone and Bones - metabolism
Cell Adhesion
Chemistry
Coated Materials, Biocompatible - chemistry
Exact sciences and technology
General and physical chemistry
Materials Testing
Osteoblasts - metabolism
Peptides - chemistry
Spectrometry, X-Ray Emission - methods
Surface physical chemistry
Surface Properties
X-Ray Film
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Summary:Immobilization of peptides on surfaces is a common method to investigate biological response to biomaterials for the development of improved tissue engineering constructs. Peptide immobilization can be achieved by either physical adsorption or covalent attachment on the surface. In this work, the RGDC peptide was covalently immobilized to alumina substrate for investigation of bone cell response. An understanding of RGDC film formation is necessary for further biological characterization. Thus, X-ray photoelectron spectroscopy was used to chemically characterize these films. These data were further utilized to calculate RGDC film thickness, density, and coverage, which are essential for understanding bone cell interactions with the underlying films and substrate.
ISSN:0743-7463
1520-5827
DOI:10.1021/la047749y