A Novel Single-Step Fabrication Technique to Create Heterogeneous Poly(ethylene glycol) Hydrogel Microstructures Containing Multiple Phenotypes of Mammalian Cells

In this study, a novel method for the one-step fabrication of stacked hydrogel microstructures using a microfluidic mold is presented. The fabrication of these structures takes advantage of the laminar flow regime in microfluidic devices, limiting the mixing of polymer precursor solutions. To create...

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Bibliographic Details
Published in:Langmuir 2005-04, Vol.21 (9), p.4168-4174
Main Authors: Zguris, Jeanna C, Itle, Laura J, Koh, Won-Gun, Pishko, Michael V
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - chemistry
Biosensing Techniques
Cell Adhesion
Cells, Cultured
Chemistry
Colloidal gels. Colloidal sols
Colloidal state and disperse state
Exact sciences and technology
Fibroblasts - chemistry
Fibroblasts - cytology
Fluorescent Dyes - chemistry
General and physical chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Isothiocyanates - chemistry
Mice
Microfluidics
Microscopy, Fluorescence
Nanostructures - chemistry
Phenotype
Polyethylene Glycols - chemistry
Rhodamines - chemistry
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Summary:In this study, a novel method for the one-step fabrication of stacked hydrogel microstructures using a microfluidic mold is presented. The fabrication of these structures takes advantage of the laminar flow regime in microfluidic devices, limiting the mixing of polymer precursor solutions. To create multilayered hydrogel structures, microfluidic devices were rotated 90° from the traditional xy axes and sealed with a cover slip. Two discreet fluidic regions form in the channels, resulting in the multilayered hydrogel upon UV polymerization. Multilayered patterned poly(ethylene glycol) hydrogel arrays (60 μm tall, 250 μm wide) containing fluorescent dyes, fluorescein isothiocyanate, and tetramethylrhodamine isothiocyanate were created for imaging purposes. Additionally, this method was used to generate hydrogel layers containing murine fibroblasts and macrophages. The cell adhesion promoter, RGD, was added to hydrogel precursor solution to enhance fibroblast cell spreading within the hydrogel matrix in one layer, but not the other. We were able to successfully generate patterns of hydrogels containing multiple phenotypes by using this technique.
ISSN:0743-7463
1520-5827
DOI:10.1021/la0470176