Effective Photocatalytic Disinfection of E. coli K-12 Using AgBr−Ag−Bi2WO6 Nanojunction System Irradiated by Visible Light: The Role of Diffusing Hydroxyl Radicals

• Published in: Environmental science & technology 2010-02, Vol.44 (4), p.1392-1398
• Main Authors: Zhang, Li-Sha, Wong, Kin-Hang, Yip, Ho-Yin, Hu, Chun, Yu, Jimmy C, Chan, Chiu-Yeung, Wong, Po-Keung
• Format: Article
• Language: English
• Subjects: Applied sciences; Bismuth - chemistry; Bromides - chemistry; Drinking water and swimming-pool water. Desalination; Escherichia coli; Escherichia coli K12 - drug effects; Escherichia coli K12 - radiation effects; Escherichia coli K12 - ultrastructure; Exact sciences and technology; Hydroxyl Radical - chemistry; Light; Microscopy, Electron, Transmission - methods; Nanostructures - chemistry; Photochemistry - methods; Pollution; Remediation and Control Technologies; Silver - chemistry; Silver Compounds - chemistry; Water treatment and pollution
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es903087w

Urgent development of effective and low-cost disinfecting technologies is needed to address the problems caused by an outbreak of harmful microorganisms. In this work, we report an effective photocatalytic disinfection of E. coli K-12 by using a AgBr−Ag−Bi2WO6 nanojunction system as a catalyst under visible light (λ ≥ 400 nm) irradiation. The visible-light-driven (VLD) AgBr−Ag−Bi2WO6 nanojunction could completely inactivate 5 × 107 cfu mL−1 E. coli K-12 within 15 min, which was superior to other VLD photocatalysts such as Bi2WO6 superstructure, Ag−Bi2WO6 and AgBr−Ag−TiO2 composite. Moreover, the photochemical mechanism of bactericidal action for the AgBr−Ag−Bi2WO6 nanojunction was investigated by using different scavengers. It was found that the diffusing hydroxyl radicals generated both by the oxidative pathway and the reductive pathway play an important role in the photocatalytic disinfection. Moreover, direct contact between the AgBr−Ag−Bi2WO6 nanojunction and bacterial cells was not necessary for the photocatalytic disinfection of E. coli K-12. Finally, the photocatalytic destruction of the bacterial cells was directly observed by TEM images and further confirmed by the determination of potassium ion (K+) leakage from the killed bacteria. This work provides a potential effective VLD photocatalyst to disinfect the bacterial cells, even to destruct the biofilm that can provide shelter and substratum for microorganisms and resist to disinfection.