Creation of Mammalian Single- and Double-Stranded DNA Surfaces:  A Real-Time QCM-D Study

The quartz crystal microbalance with dissipation monitoring (QCM-D) is an excellent method for studying the creation of DNA-based surfaces and films. Previous studies have used QCM-D to focus on the construction of DNA surfaces composed of short synthetic DNA oligomers or plasmid DNA. Here, we have...

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Bibliographic Details
Published in:Langmuir 2007-09, Vol.23 (19), p.9563-9566
Main Authors: Rawle, Robert J, Selassie, Cynthia R.D, Johal, Malkiat S
Format: Article
Language:English
Subjects:
Animals
Chemistry
Colloidal state and disperse state
DNA - chemistry
Exact sciences and technology
General and physical chemistry
Humans
Ligands
Models, Biological
Polyamines - chemistry
Quartz
Silicon - chemistry
Surface physical chemistry
Surface Properties
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Summary:The quartz crystal microbalance with dissipation monitoring (QCM-D) is an excellent method for studying the creation of DNA-based surfaces and films. Previous studies have used QCM-D to focus on the construction of DNA surfaces composed of short synthetic DNA oligomers or plasmid DNA. Here, we have used QCM-D to monitor the creation of genomic single- and double-stranded calf thymus DNA surfaces on a polycation adsorbed to a SiO2 support. We have successfully monitored the hybridization between the ssDNA surfaces and their complementary strands in solution and have also shown that DNA multilayer formation can be observed using denatured calf thymus DNA. We have furthermore established that the ssDNA and dsDNA surfaces show different binding characteristics to ethidium bromide, a common dsDNA intercalator, demonstrating the potential use of such surfaces to identify possible DNA ligands.
ISSN:0743-7463
1520-5827
DOI:10.1021/la702043t