Synthesis and Characterization of Core–Shell Star Copolymers for In Vivo PET Imaging Applications

The synthesis of core–shell star copolymers via living free radical polymerization provides a convenient route to three-dimensional nanostructures having a poly(ethylene glycol) outer shell, a hydrophilic inner shell bearing reactive functional groups, and a central hydrophobic core. By starting wit...

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Bibliographic Details
Published in:Bioconjugate chemistry 2008-04, Vol.9 (4), p.1329-1339
Main Authors: Fukukawa, Ken-ichi, Rossin, Raffaella, Hagooly, Aviv, Pressly, Eric D, Hunt, Jasmine N, Messmore, Benjamin W, Hawker, Craig J
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Biological and medical sciences
Copper Radioisotopes
Drug Carriers
Exact sciences and technology
Female
Heterocyclic Compounds, 1-Ring - metabolism
Investigative techniques, diagnostic techniques (general aspects)
Magnetic Resonance Spectroscopy
Medical sciences
Miscellaneous. Technology
Nanoparticles
Organic polymers
Physicochemistry of polymers
Polyethylene Glycols - chemistry
Polymers - chemical synthesis
Polymers - chemistry
Polymers - metabolism
Polymers with particular properties
Positron-Emission Tomography
Preparation, kinetics, thermodynamics, mechanism and catalysts
Radionuclide investigations
Rats
Rats, Sprague-Dawley
Surface Properties
Tissue Distribution
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Summary:The synthesis of core–shell star copolymers via living free radical polymerization provides a convenient route to three-dimensional nanostructures having a poly(ethylene glycol) outer shell, a hydrophilic inner shell bearing reactive functional groups, and a central hydrophobic core. By starting with well-defined linear diblock copolymers, the thickness of each layer, overall size/molecular weight, and the number of internal reactive functional groups can be controlled accurately, permitting detailed structure/performance information to be obtained. Functionalization of these polymeric nanoparticles with a DOTA-ligand capable of chelating radioactive 64Cu nuclei enabled the biodistribution and in vivo positron emission tomography (PET) imaging of these materials to be studied and correlated directly to the initial structure. Results indicate that nanoparticles with increasing PEG shell thickness show increased blood circulation and low accumulation in excretory organs, suggesting application as in vivo carriers for imaging, targeting, and therapeutic groups.
ISSN:1525-7797
1043-1802
1526-4602
1520-4812
DOI:10.1021/bm7014152