Rapid Raman Imaging of Stable, Functionalized Nanoshells in Mammalian Cell Cultures

Two Raman-active poly(ethylene glycol) (PEG) molecules, one linear (MW 5000) and the other branched (MW 2420), are synthesized to stabilize gold-silica nanoshells in cell culture media and track nanoparticles in mammalian cell cultures. The linear PEG provides greater nanoshell stability in saline s...

Full description

Saved in:
Bibliographic Details
Published in:Nano letters 2009-08, Vol.9 (8), p.2914-2920
Main Authors: Huang, Yiming, Swarup, Vimal P, Bishnoi, Sandra Whaley
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Condensed matter: structure, mechanical and thermal properties
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
Gold - chemistry
Humans
Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties
Materials science
Metal Nanoparticles - chemistry
Mice
Molecular Structure
Nanocrystalline materials
Nanoscale materials and structures: fabrication and characterization
Physics
Polyethylene Glycols - chemistry
Spectrum Analysis, Raman
Surfaces and interfaces
thin films and whiskers (structure and nonelectronic properties)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two Raman-active poly(ethylene glycol) (PEG) molecules, one linear (MW 5000) and the other branched (MW 2420), are synthesized to stabilize gold-silica nanoshells in cell culture media and track nanoparticles in mammalian cell cultures. The linear PEG provides greater nanoshell stability in saline solution compared to commercially available PEG-thiol or the branched PEG. Surface enhanced Raman scattering rapidly tracks the probes and provides semiquantitative information regarding particle localization within mouse macrophage (RAW 264.7) and human breast cancer (MCF 7) cell cultures.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl901234x