Tea polyphenols inhibit IL-6 production in tumor necrosis factor superfamily 14-stimulated human gingival fibroblasts

• Published in: Molecular nutrition & food research 2010-07, Vol.54 (S2), p.S151-S158
• Main Authors: Hosokawa, Yoshitaka, Hosokawa, Ikuko, Ozaki, Kazumi, Nakanishi, Tadashi, Nakae, Hideaki, Matsuo, Takashi
• Format: Article
• Language: English
• Subjects: Aged; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Antioxidants - pharmacology; Biflavonoids - pharmacology; Catechin - analogs & derivatives; Catechin - pharmacology; Catechins; Cells, Cultured; Chronic Periodontitis - drug therapy; Chronic Periodontitis - metabolism; Enzyme Inhibitors - pharmacology; Female; Fibroblasts - drug effects; Fibroblasts - metabolism; Flavonoids - pharmacology; Gallic Acid - analogs & derivatives; Gallic Acid - pharmacology; Gene Expression Regulation - drug effects; Gingiva - cytology; Gingiva - drug effects; Gingiva - metabolism; Human gingival fibroblasts; Humans; IL-6; Interleukin-6 - metabolism; Interleukin-6 - secretion; Male; Middle Aged; Phenols - pharmacology; Polyphenols; RNA, Messenger - metabolism; Signal Transduction - drug effects; Tea - chemistry; Theaflavins; Tumor Necrosis Factor Ligand Superfamily Member 14 - genetics; Tumor Necrosis Factor Ligand Superfamily Member 14 - physiology; Tumor necrosis factor superfamily 14
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.200900549

IL-6 is well recognized to be a potent bone resorptive agent and thus in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins in green tea, and theaflavin-3,3′-digallate (TFDG), polyphenol in black tea, have multiple beneficial effects, but the effects of catechins and theaflavins on IL-6 production in human gingival fibroblasts (HGFs) are not known. In this study, we investigated the mechanisms by which EGCG, ECG, and TFDG inhibit tumor necrosis factor superfamily 14 (TNFSF14)-induced IL-6 production in HGFs. We detected TNFSF14 mRNA expression in human diseased periodontal tissues. TNFSF14 increased IL-6 production in HGFs in a concentration-dependent manner. EGCG, ECG, and TFDG prevented TNFSF14-mediated IL-6 production in HGFs. EGCG, ECG, and TFDG prevented TNFSF14-induced extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and nuclear factor-κB activation in HGFs. Inhibitors of ERK, JNK, and nuclear factor-κB decreased TNFSF14-induced IL-6 production. In addition, EGCG, ECG, and TFDG attenuated TNFSF14 receptor expression on HGFs. These data provide a novel mechanism through which the green tea and black tea polyphenols could be used to provide direct benefits in periodontal disease.