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Parenteral lipids impair pneumococcal elimination by human neutrophils
Eur J Clin Invest 2010; 40 (8): 729–734 Background Lipid‐induced modulation of phagocyte function seems to contribute to increased susceptibility to infections in patients on parenteral nutrition, and an increased risk for development of pneumonia has been observed in this group. The role of variou...
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| Published in: | European journal of clinical investigation 2010-08, Vol.40 (8), p.729-734 |
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| creator | Versleijen, Michelle W. Roelofs, Hennie M. Te Morsche, Rene H. Simonetti, Elles R. Hermans, Peter W. Wanten, Geert J. |
| description | Eur J Clin Invest 2010; 40 (8): 729–734
Background Lipid‐induced modulation of phagocyte function seems to contribute to increased susceptibility to infections in patients on parenteral nutrition, and an increased risk for development of pneumonia has been observed in this group. The role of various structurally different lipid emulsions, however, remains unclear. In this study, we therefore assessed phagocyte function, as the capacity of neutrophils to eliminate Streptococcus pneumoniae (i.e. combined result of phagocytosis and killing), in the presence of these lipids.
Materials and methods Neutrophils from six healthy volunteers were incubated for 1 h in emulsions (5 mmol L−1) derived from soybean‐ (LCT), fish‐ (VLCT), olive‐ (LCT‐MUFA), mixed soybean/coconut oils (LCT/MCTs) or structured lipids (SL). After opsonization of the pneumococci (strain OREP‐4) by human immunoglobulins, bacteria and neutrophils were incubated in the presence of complement. Next, pneumococcal elimination was evaluated and expressed as the percentage of bacteria eliminated relative to the initial bacterial numbers in neutrophil‐free samples.
Results Neutrophils that were not exposed to lipids showed a pneumococcal elimination capacity of 75 ± 3% (mean ± SD). This significantly decreased after exposure to LCT‐MUFA (70 ± 6%), VLCT (67 ± 2%), SL (63 ± 9%), LCT (66 ± 10%) and LCT/MCT (47 ± 15%).
Conclusion These data demonstrate that parenteral lipids impair the microbial elimination capacity of neutrophils in a structure‐dependent manner. In accordance with our previously reported in vitro effect on a range of phagocyte functions, LCT/MCT is by far the most potent in this respect. |
| doi_str_mv | 10.1111/j.1365-2362.2010.02320.x |
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| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_754558181</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>754558181</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4680-5286f1521e721b8400c77f9ff3e13d1fc8363bd2aa32a0ef3cd514a649ed27793</originalsourceid><addsrcrecordid>eNqNkE1vEzEQhi0EoqHwF9BeEKdNZ-y1vXvggKI2rVSVHoLgZjleW3XYL-xdNfn3eEkIR_DFlud5Z-yHkAxhiWld7ZbIBM8pE3RJId0CZRSW-xdkcS68JAsALHJaSXpB3sS4A4ASGX1NLihwgcBwQW4edbDdaINussYPvo6ZbwftQzZ0dmp70xuTSrbxre_06Psu2x6yp6nVXZaAMfTDk2_iW_LK6Sbad6f9kny9ud6sbvP7L-u71ef73BSihJzTUjjkFK2kuC0LACOlq5xjFlmNzpRMsG1NtWZUg3XM1BwLLYrK1lTKil2Sj8e-Q-h_TjaOqvXR2KbRne2nqCQvOC8xffOfJEvTRYkikeWRNKGPMVinhuBbHQ4KQc261U7NVtVsVc261W_dap-i709Dpm1r63Pwj98EfDgBOiaPLujO-PiXo1UFAljiPh25Z9_Yw38_QF2v7uZTyufHvI-j3Z_zOvxQQjLJ1beHtbrdbNaIj9_Vmv0CQb2pWA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734006816</pqid></control><display><type>article</type><title>Parenteral lipids impair pneumococcal elimination by human neutrophils</title><source>Wiley</source><creator>Versleijen, Michelle W. ; Roelofs, Hennie M. ; Te Morsche, Rene H. ; Simonetti, Elles R. ; Hermans, Peter W. ; Wanten, Geert J.</creator><creatorcontrib>Versleijen, Michelle W. ; Roelofs, Hennie M. ; Te Morsche, Rene H. ; Simonetti, Elles R. ; Hermans, Peter W. ; Wanten, Geert J.</creatorcontrib><description>Eur J Clin Invest 2010; 40 (8): 729–734
Background Lipid‐induced modulation of phagocyte function seems to contribute to increased susceptibility to infections in patients on parenteral nutrition, and an increased risk for development of pneumonia has been observed in this group. The role of various structurally different lipid emulsions, however, remains unclear. In this study, we therefore assessed phagocyte function, as the capacity of neutrophils to eliminate Streptococcus pneumoniae (i.e. combined result of phagocytosis and killing), in the presence of these lipids.
Materials and methods Neutrophils from six healthy volunteers were incubated for 1 h in emulsions (5 mmol L−1) derived from soybean‐ (LCT), fish‐ (VLCT), olive‐ (LCT‐MUFA), mixed soybean/coconut oils (LCT/MCTs) or structured lipids (SL). After opsonization of the pneumococci (strain OREP‐4) by human immunoglobulins, bacteria and neutrophils were incubated in the presence of complement. Next, pneumococcal elimination was evaluated and expressed as the percentage of bacteria eliminated relative to the initial bacterial numbers in neutrophil‐free samples.
Results Neutrophils that were not exposed to lipids showed a pneumococcal elimination capacity of 75 ± 3% (mean ± SD). This significantly decreased after exposure to LCT‐MUFA (70 ± 6%), VLCT (67 ± 2%), SL (63 ± 9%), LCT (66 ± 10%) and LCT/MCT (47 ± 15%).
Conclusion These data demonstrate that parenteral lipids impair the microbial elimination capacity of neutrophils in a structure‐dependent manner. In accordance with our previously reported in vitro effect on a range of phagocyte functions, LCT/MCT is by far the most potent in this respect.</description><identifier>ISSN: 0014-2972</identifier><identifier>EISSN: 1365-2362</identifier><identifier>DOI: 10.1111/j.1365-2362.2010.02320.x</identifier><identifier>PMID: 20561031</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Bacterial diseases ; Biological and medical sciences ; Cells, Cultured ; Colony Count, Microbial ; Data processing ; Female ; Fish Oils ; General aspects ; Human ; Human bacterial diseases ; Humans ; immune modulation ; Immunoglobulins ; Infection ; Infectious diseases ; Leukocytes (neutrophilic) ; Lipids ; Lipids - pharmacology ; Male ; Medical sciences ; neutrophil ; Neutrophils - drug effects ; Neutrophils - physiology ; Oils ; Opsonization ; parenteral lipid ; Parenteral nutrition ; Phagocytes ; Phagocytosis ; Phagocytosis - drug effects ; Plant Extracts ; pneumococcal survival ; Pneumonia ; Staphylococcal infections, streptococcal infections, pneumococcal infections ; Streptococcus pneumoniae ; Streptococcus pneumoniae - physiology ; Young Adult</subject><ispartof>European journal of clinical investigation, 2010-08, Vol.40 (8), p.729-734</ispartof><rights>2010 The Authors. Journal Compilation © 2010 Stichting European Society for Clinical Investigation Journal Foundation</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4680-5286f1521e721b8400c77f9ff3e13d1fc8363bd2aa32a0ef3cd514a649ed27793</citedby><cites>FETCH-LOGICAL-c4680-5286f1521e721b8400c77f9ff3e13d1fc8363bd2aa32a0ef3cd514a649ed27793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22990603$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20561031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Versleijen, Michelle W.</creatorcontrib><creatorcontrib>Roelofs, Hennie M.</creatorcontrib><creatorcontrib>Te Morsche, Rene H.</creatorcontrib><creatorcontrib>Simonetti, Elles R.</creatorcontrib><creatorcontrib>Hermans, Peter W.</creatorcontrib><creatorcontrib>Wanten, Geert J.</creatorcontrib><title>Parenteral lipids impair pneumococcal elimination by human neutrophils</title><title>European journal of clinical investigation</title><addtitle>Eur J Clin Invest</addtitle><description>Eur J Clin Invest 2010; 40 (8): 729–734
Background Lipid‐induced modulation of phagocyte function seems to contribute to increased susceptibility to infections in patients on parenteral nutrition, and an increased risk for development of pneumonia has been observed in this group. The role of various structurally different lipid emulsions, however, remains unclear. In this study, we therefore assessed phagocyte function, as the capacity of neutrophils to eliminate Streptococcus pneumoniae (i.e. combined result of phagocytosis and killing), in the presence of these lipids.
Materials and methods Neutrophils from six healthy volunteers were incubated for 1 h in emulsions (5 mmol L−1) derived from soybean‐ (LCT), fish‐ (VLCT), olive‐ (LCT‐MUFA), mixed soybean/coconut oils (LCT/MCTs) or structured lipids (SL). After opsonization of the pneumococci (strain OREP‐4) by human immunoglobulins, bacteria and neutrophils were incubated in the presence of complement. Next, pneumococcal elimination was evaluated and expressed as the percentage of bacteria eliminated relative to the initial bacterial numbers in neutrophil‐free samples.
Results Neutrophils that were not exposed to lipids showed a pneumococcal elimination capacity of 75 ± 3% (mean ± SD). This significantly decreased after exposure to LCT‐MUFA (70 ± 6%), VLCT (67 ± 2%), SL (63 ± 9%), LCT (66 ± 10%) and LCT/MCT (47 ± 15%).
Conclusion These data demonstrate that parenteral lipids impair the microbial elimination capacity of neutrophils in a structure‐dependent manner. In accordance with our previously reported in vitro effect on a range of phagocyte functions, LCT/MCT is by far the most potent in this respect.</description><subject>Adult</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Colony Count, Microbial</subject><subject>Data processing</subject><subject>Female</subject><subject>Fish Oils</subject><subject>General aspects</subject><subject>Human</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>immune modulation</subject><subject>Immunoglobulins</subject><subject>Infection</subject><subject>Infectious diseases</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lipids</subject><subject>Lipids - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>neutrophil</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - physiology</subject><subject>Oils</subject><subject>Opsonization</subject><subject>parenteral lipid</subject><subject>Parenteral nutrition</subject><subject>Phagocytes</subject><subject>Phagocytosis</subject><subject>Phagocytosis - drug effects</subject><subject>Plant Extracts</subject><subject>pneumococcal survival</subject><subject>Pneumonia</subject><subject>Staphylococcal infections, streptococcal infections, pneumococcal infections</subject><subject>Streptococcus pneumoniae</subject><subject>Streptococcus pneumoniae - physiology</subject><subject>Young Adult</subject><issn>0014-2972</issn><issn>1365-2362</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkE1vEzEQhi0EoqHwF9BeEKdNZ-y1vXvggKI2rVSVHoLgZjleW3XYL-xdNfn3eEkIR_DFlud5Z-yHkAxhiWld7ZbIBM8pE3RJId0CZRSW-xdkcS68JAsALHJaSXpB3sS4A4ASGX1NLihwgcBwQW4edbDdaINussYPvo6ZbwftQzZ0dmp70xuTSrbxre_06Psu2x6yp6nVXZaAMfTDk2_iW_LK6Sbad6f9kny9ud6sbvP7L-u71ef73BSihJzTUjjkFK2kuC0LACOlq5xjFlmNzpRMsG1NtWZUg3XM1BwLLYrK1lTKil2Sj8e-Q-h_TjaOqvXR2KbRne2nqCQvOC8xffOfJEvTRYkikeWRNKGPMVinhuBbHQ4KQc261U7NVtVsVc261W_dap-i709Dpm1r63Pwj98EfDgBOiaPLujO-PiXo1UFAljiPh25Z9_Yw38_QF2v7uZTyufHvI-j3Z_zOvxQQjLJ1beHtbrdbNaIj9_Vmv0CQb2pWA</recordid><startdate>201008</startdate><enddate>201008</enddate><creator>Versleijen, Michelle W.</creator><creator>Roelofs, Hennie M.</creator><creator>Te Morsche, Rene H.</creator><creator>Simonetti, Elles R.</creator><creator>Hermans, Peter W.</creator><creator>Wanten, Geert J.</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope></search><sort><creationdate>201008</creationdate><title>Parenteral lipids impair pneumococcal elimination by human neutrophils</title><author>Versleijen, Michelle W. ; Roelofs, Hennie M. ; Te Morsche, Rene H. ; Simonetti, Elles R. ; Hermans, Peter W. ; Wanten, Geert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4680-5286f1521e721b8400c77f9ff3e13d1fc8363bd2aa32a0ef3cd514a649ed27793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Colony Count, Microbial</topic><topic>Data processing</topic><topic>Female</topic><topic>Fish Oils</topic><topic>General aspects</topic><topic>Human</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>immune modulation</topic><topic>Immunoglobulins</topic><topic>Infection</topic><topic>Infectious diseases</topic><topic>Leukocytes (neutrophilic)</topic><topic>Lipids</topic><topic>Lipids - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>neutrophil</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - physiology</topic><topic>Oils</topic><topic>Opsonization</topic><topic>parenteral lipid</topic><topic>Parenteral nutrition</topic><topic>Phagocytes</topic><topic>Phagocytosis</topic><topic>Phagocytosis - drug effects</topic><topic>Plant Extracts</topic><topic>pneumococcal survival</topic><topic>Pneumonia</topic><topic>Staphylococcal infections, streptococcal infections, pneumococcal infections</topic><topic>Streptococcus pneumoniae</topic><topic>Streptococcus pneumoniae - physiology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Versleijen, Michelle W.</creatorcontrib><creatorcontrib>Roelofs, Hennie M.</creatorcontrib><creatorcontrib>Te Morsche, Rene H.</creatorcontrib><creatorcontrib>Simonetti, Elles R.</creatorcontrib><creatorcontrib>Hermans, Peter W.</creatorcontrib><creatorcontrib>Wanten, Geert J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>European journal of clinical investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Versleijen, Michelle W.</au><au>Roelofs, Hennie M.</au><au>Te Morsche, Rene H.</au><au>Simonetti, Elles R.</au><au>Hermans, Peter W.</au><au>Wanten, Geert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Parenteral lipids impair pneumococcal elimination by human neutrophils</atitle><jtitle>European journal of clinical investigation</jtitle><addtitle>Eur J Clin Invest</addtitle><date>2010-08</date><risdate>2010</risdate><volume>40</volume><issue>8</issue><spage>729</spage><epage>734</epage><pages>729-734</pages><issn>0014-2972</issn><eissn>1365-2362</eissn><abstract>Eur J Clin Invest 2010; 40 (8): 729–734
Background Lipid‐induced modulation of phagocyte function seems to contribute to increased susceptibility to infections in patients on parenteral nutrition, and an increased risk for development of pneumonia has been observed in this group. The role of various structurally different lipid emulsions, however, remains unclear. In this study, we therefore assessed phagocyte function, as the capacity of neutrophils to eliminate Streptococcus pneumoniae (i.e. combined result of phagocytosis and killing), in the presence of these lipids.
Materials and methods Neutrophils from six healthy volunteers were incubated for 1 h in emulsions (5 mmol L−1) derived from soybean‐ (LCT), fish‐ (VLCT), olive‐ (LCT‐MUFA), mixed soybean/coconut oils (LCT/MCTs) or structured lipids (SL). After opsonization of the pneumococci (strain OREP‐4) by human immunoglobulins, bacteria and neutrophils were incubated in the presence of complement. Next, pneumococcal elimination was evaluated and expressed as the percentage of bacteria eliminated relative to the initial bacterial numbers in neutrophil‐free samples.
Results Neutrophils that were not exposed to lipids showed a pneumococcal elimination capacity of 75 ± 3% (mean ± SD). This significantly decreased after exposure to LCT‐MUFA (70 ± 6%), VLCT (67 ± 2%), SL (63 ± 9%), LCT (66 ± 10%) and LCT/MCT (47 ± 15%).
Conclusion These data demonstrate that parenteral lipids impair the microbial elimination capacity of neutrophils in a structure‐dependent manner. In accordance with our previously reported in vitro effect on a range of phagocyte functions, LCT/MCT is by far the most potent in this respect.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20561031</pmid><doi>10.1111/j.1365-2362.2010.02320.x</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Bacterial diseases Biological and medical sciences Cells, Cultured Colony Count, Microbial Data processing Female Fish Oils General aspects Human Human bacterial diseases Humans immune modulation Immunoglobulins Infection Infectious diseases Leukocytes (neutrophilic) Lipids Lipids - pharmacology Male Medical sciences neutrophil Neutrophils - drug effects Neutrophils - physiology Oils Opsonization parenteral lipid Parenteral nutrition Phagocytes Phagocytosis Phagocytosis - drug effects Plant Extracts pneumococcal survival Pneumonia Staphylococcal infections, streptococcal infections, pneumococcal infections Streptococcus pneumoniae Streptococcus pneumoniae - physiology Young Adult |
| title | Parenteral lipids impair pneumococcal elimination by human neutrophils |
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