Production of L-malic acid by permeabilized cells of commercial Saccharomyces sp. strains

Of various yeasts tested in the conversion of fumaric to L-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times...

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Bibliographic Details
Published in:Biotechnology letters 2005-12, Vol.27 (23-24), p.1835-1839
Main Authors: VRSALOVIC PRESECKI, Ana, VASIE-RACKI, Durda
Format: Article
Language:English
Subjects:
Bacteria
Batch production
Biological and medical sciences
Biotechnology
Cell Membrane Permeability - drug effects
Cell Membrane Permeability - physiology
Cetrimonium Compounds - pharmacology
Conversion
Enzymatic activity
Fumarate Hydratase - metabolism
Fumarates - metabolism
Fumaric acid
Fundamental and applied biological sciences. Psychology
Growth conditions
Kinetics
Malates - metabolism
Mathematical models
Microbiology
Saccharomyces
Saccharomyces - drug effects
Saccharomyces - enzymology
Saccharomyces - metabolism
Saccharomyces bayanus
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - metabolism
Species Specificity
Strain
Surface-Active Agents - pharmacology
Time Factors
Yeasts
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Summary:Of various yeasts tested in the conversion of fumaric to L-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of L-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82% and gave 21, 40, 83 and 175 mM L-malic acid from respectively, 25, 50, 100 and 210 mM: fumaric acid.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-005-3890-3