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Production of L-malic acid by permeabilized cells of commercial Saccharomyces sp. strains

Of various yeasts tested in the conversion of fumaric to L-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times...

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Published in:	Biotechnology letters 2005-12, Vol.27 (23-24), p.1835-1839
Main Authors:	VRSALOVIC PRESECKI, Ana, VASIE-RACKI, Durda
Format:	Article
Language:	English
Subjects:	Bacteria Batch production Biological and medical sciences Biotechnology Cell Membrane Permeability - drug effects Cell Membrane Permeability - physiology Cetrimonium Compounds - pharmacology Conversion Enzymatic activity Fumarate Hydratase - metabolism Fumarates - metabolism Fumaric acid Fundamental and applied biological sciences. Psychology Growth conditions Kinetics Malates - metabolism Mathematical models Microbiology Saccharomyces Saccharomyces - drug effects Saccharomyces - enzymology Saccharomyces - metabolism Saccharomyces bayanus Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - metabolism Species Specificity Strain Surface-Active Agents - pharmacology Time Factors Yeasts
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creator	VRSALOVIC PRESECKI, Ana VASIE-RACKI, Durda
description	Of various yeasts tested in the conversion of fumaric to L-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of L-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82% and gave 21, 40, 83 and 175 mM L-malic acid from respectively, 25, 50, 100 and 210 mM: fumaric acid.
doi_str_mv	10.1007/s10529-005-3890-3
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Psychology ; Growth conditions ; Kinetics ; Malates - metabolism ; Mathematical models ; Microbiology ; Saccharomyces ; Saccharomyces - drug effects ; Saccharomyces - enzymology ; Saccharomyces - metabolism ; Saccharomyces bayanus ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - metabolism ; Species Specificity ; Strain ; Surface-Active Agents - pharmacology ; Time Factors ; Yeasts</subject><ispartof>Biotechnology letters, 2005-12, Vol.27 (23-24), p.1835-1839</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-44c25a86b86a672b5d610d977e370b6b6af13e4cc6a80285b36f3f66c9a898f13</citedby><cites>FETCH-LOGICAL-c484t-44c25a86b86a672b5d610d977e370b6b6af13e4cc6a80285b36f3f66c9a898f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17348994$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16328976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VRSALOVIC PRESECKI, Ana</creatorcontrib><creatorcontrib>VASIE-RACKI, Durda</creatorcontrib><title>Production of L-malic acid by permeabilized cells of commercial Saccharomyces sp. strains</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>Of various yeasts tested in the conversion of fumaric to L-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of L-malic acid was validated at different initial fumaric acid concentrations. 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Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of L-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82% and gave 21, 40, 83 and 175 mM L-malic acid from respectively, 25, 50, 100 and 210 mM: fumaric acid.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>16328976</pmid><doi>10.1007/s10529-005-3890-3</doi><tpages>5</tpages></addata></record>
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subjects	Bacteria Batch production Biological and medical sciences Biotechnology Cell Membrane Permeability - drug effects Cell Membrane Permeability - physiology Cetrimonium Compounds - pharmacology Conversion Enzymatic activity Fumarate Hydratase - metabolism Fumarates - metabolism Fumaric acid Fundamental and applied biological sciences. Psychology Growth conditions Kinetics Malates - metabolism Mathematical models Microbiology Saccharomyces Saccharomyces - drug effects Saccharomyces - enzymology Saccharomyces - metabolism Saccharomyces bayanus Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - metabolism Species Specificity Strain Surface-Active Agents - pharmacology Time Factors Yeasts
title	Production of L-malic acid by permeabilized cells of commercial Saccharomyces sp. strains
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