Raffinose-hydrolyzing activity of Aspergillus fumigatus

Aspergillus fumigatus can utilize raffinose and melibiose as its sole carbon source. Substantial extracellular raffinolytic and invertase activities were produced during fungal growth on raffinose. These activities were induced also by sucrose, melibiose and wheat straw but not by glucose. Hydrolysi...

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Bibliographic Details
Published in:Biotechnology letters 1997-03, Vol.19 (3), p.217-220
Main Authors: Rezende, S.T. de, Felix, C.R
Format: Article
Language:English
Subjects:
Aspergillus fumigatus
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
feed processing
food microbiology
food processing
Fundamental and applied biological sciences. Psychology
microbiology
Mission oriented research
Physiology and metabolism
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Summary:Aspergillus fumigatus can utilize raffinose and melibiose as its sole carbon source. Substantial extracellular raffinolytic and invertase activities were produced during fungal growth on raffinose. These activities were induced also by sucrose, melibiose and wheat straw but not by glucose. Hydrolysis of both raffinose and sucrose was optimal at pH 4.5 to 5.5 and 55 to 60 degrees C. Melibiose was also hydrolyzed by the culture filtrate. The crude enzyme retained about 80% of the original activity (2 U/mL) after incubation for 2 h at 50 degrees C.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1018393221180