Dextran-spermine conjugate: an efficient vector for gene delivery

Cationic Polysaccharides based on oligoamine‐dextran conjugates were synthesized and tested as vectors for gene transfection. Dextran with 40 kDa in average molecular weight was oxidized under mild conditions by potassium periodate to obtain the respective polyaldehydes in relatively high yields (∼9...

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Bibliographic Details
Published in:Macromolecular symposia. 2003-07, Vol.195 (1), p.247-262
Main Authors: Azzam, T., Eliyahu, H., Makovitzki, A., Domb, A.J.
Format: Article
Language:English
Subjects:
dextran
polyamines
polyelectrolytes
reductive-amination
spermine
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Summary:Cationic Polysaccharides based on oligoamine‐dextran conjugates were synthesized and tested as vectors for gene transfection. Dextran with 40 kDa in average molecular weight was oxidized under mild conditions by potassium periodate to obtain the respective polyaldehydes in relatively high yields (∼90%). The oxidized dextran was reacted by reductive animation with various oligoamines of 2 to 4 amino groups to obtain the corresponding imine‐conjugates. These water‐soluble polymers were then reduced by excess of sodium borohydride to obtain the corresponding amine‐conjuagtes in 30‐40% overall yield. The electrostatic interactions of the representative polycations with plasmid DNA were evaluated as a function of charge ratio (+/−, polymer/DNA) and ionic strength of the medium applying the ethidium‐bromide quenching assay. Although most synthetic polycations formed stable complexes with Plasmid DNAs, only the dextran‐spermine conjugate of a defined amino content and molecular weight was able to transfect cells with high efficiency.
ISSN:1022-1360
1521-3900
DOI:10.1002/masy.200390130