Modified vaccinia Ankara strains with identical coding sequences actually represent complex mixtures of viruses that determine the biological properties of each strain

AbstractModified vaccinia Ankara (MVA) was developed by serial passages on chicken embryo fibroblast cells. After passage 570, the virus was considered homogenous and genetically stable. Three MVA strains (MVA-572, MVA-I721 and MVA-BN ®) have been analyzed and shown to be 100% genetically identical;...

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Bibliographic Details
Published in:Vaccine 2009-12, Vol.27 (52), p.7442-7450
Main Authors: Suter, Mark, Meisinger-Henschel, Christine, Tzatzaris, Maria, Hülsemann, Vanessa, Lukassen, Susanne, Wulff, Niels Holger, Hausmann, Jürgen, Howley, Paul, Chaplin, Paul
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Applied microbiology
Biological and medical sciences
Biological heterogeneity
Biological properties
Chick Embryo
Deoxyribonucleic acid
DNA
DNA, Viral - analysis
Embryos
Female
Fundamental and applied biological sciences. Psychology
Genome, Viral
Genomes
HeLa Cells
Humans
Mammals
Mice
Mice, Knockout
Microbiology
MVA
Ovary - virology
Phenotype
Sequence identity
Serial Passage
Studies
Subpopulations
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccinia virus - genetics
Vaccinia virus - physiology
Virus Cultivation
Virus Replication
Viruses
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Summary:AbstractModified vaccinia Ankara (MVA) was developed by serial passages on chicken embryo fibroblast cells. After passage 570, the virus was considered homogenous and genetically stable. Three MVA strains (MVA-572, MVA-I721 and MVA-BN ®) have been analyzed and shown to be 100% genetically identical; although significant differences in their phenotypes were illustrated. All MVA strains except MVA-BN ® replicated in human cells, or killed immune suppressed mice. Viruses isolated from dead animals were shown to represent variants present within MVA-572 or MVA-I721 used to inoculate the mice. These subpopulations were shown to encode mutations, or contain less than the six deletions associated with MVA and had significantly altered phenotypes compared to the parental MVA strains. MVA is a complex polyclonal mixture of viruses, the composition of which governs the phenotype.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2009.05.095