Plasmodium vivax hypoxanthine-guanine phosphoribosyltransferase: A target for anti-malarial chemotherapy

• Published in: Molecular and biochemical parasitology 2010-10, Vol.173 (2), p.165-169
• Main Authors: Keough, Dianne T., Hocková, Dana, Krečmerová, Marcela, Česnek, Michal, Holý, Antonín, Naesens, Lieve, Brereton, Ian M., Winzor, Donald J., de Jersey, John, Guddat, Luke W.
• Format: Article
• Language: English
• Subjects: 5-phospho-α-d-ribosyl-1-pyrophosphate; 6-Oxopurine phosphoribosyltransferase; acyclic nucleoside phosphonate; Acyclic nucleoside phosphonates; ANP; Coenzymes - metabolism; dithiothreitol; DTT; Enzyme Inhibitors - metabolism; Guanine - metabolism; HGPRT; Humans; Hypoxanthine - metabolism; Hypoxanthine Phosphoribosyltransferase - genetics; Hypoxanthine Phosphoribosyltransferase - isolation & purification; Hypoxanthine Phosphoribosyltransferase - metabolism; hypoxanthine-guanine phosphoribosyltransferase; Magnesium - metabolism; Mass Spectrometry; Organophosphonates - metabolism; PfHGXPRT; phosphoribosyltransferase; Plasmodium falciparum - enzymology; Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase; Plasmodium vivax; Plasmodium vivax - enzymology; Plasmodium vivax hypoxanthine-guanine-phosphoribosyltransferase; PPi; PRib-PP; Protein Binding; PRTase; Purine salvage pathway; PvHGPRT; pyrophosphate; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Substrate Specificity
• ISSN: 0166-6851; 1872-9428
• DOI: 10.1016/j.molbiopara.2010.05.018

Plasmodium vivax causes a severe form of malaria. A target for new chemotherapeutics is hypoxanthine-guanine phosporibosyltransferase. This enzyme has been characterized and inhibitors discovered. The malarial parasite, Plasmodium vivax (Pv), causes a serious infectious disease found primarily in Asia and the Americas. For protozoan parasites, 6-oxopurine phosphoribosyltransferases (PRTases) provide the only metabolic pathway to synthesize the purine nucleoside monophosphates essential for DNA/RNA production. We have purified the recombinant Pv 6-oxopurine (PRTase) and compared its properties with the human and Pf enzymes. The Pv enzyme uses hypoxanthine and guanine with similar catalytic efficiency to the Pf enzyme but xanthine is not a substrate, hence we identify this enzyme as PvHGPRT. Mass spectrometry suggests that PvHGPRT contains bound magnesium ions that are removed by EDTA resulting in loss of activity. However, the addition of Mg2+ restores activity. Acyclic nucleoside phosphonates (ANPs) are good inhibitors of PvHGPRT having Ki values as low as 3μM. These compounds can form the basis for the design of new drugs aimed at combating malaria caused by Pv.