Increased thermostability of three mesophilic b-galactosidases under high pressure

High pressure (>200Mpa) or high temperature (>45C) can both induce an irreversible inactivation of the b-galactosidases of Aspergillus oryzae, Kluyveromyces lactis and Escherichia coli. Moderate pressures (50MPa-250 MPa) exerted a protective effect against thermal inactivation for the three b-...

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Bibliographic Details
Published in:Biotechnology letters 1997-03, Vol.19 (3), p.273-276
Main Authors: Athes, Violaine, Degraeve, Pascal, Cavaille-Lefebvre, Dominique, Espeillac, Sandrine, Lemay, Pierre, Combes, Didier
Format: Article
Language:English
Subjects:
Aspergillus oryzae
Biotechnology
Escherichia coli
Hydrolysis
Inactivation
Kluyveromyces lactis
Lactose
Protective
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Summary:High pressure (>200Mpa) or high temperature (>45C) can both induce an irreversible inactivation of the b-galactosidases of Aspergillus oryzae, Kluyveromyces lactis and Escherichia coli. Moderate pressures (50MPa-250 MPa) exerted a protective effect against thermal inactivation for the three b-galactosidases investigated. High pressure could thus be used to carry out b-galactosidase catalysed reactions such as lactose hydrolysis, at higher temperatures.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1018317926631