Protective efficacy of bacterial membranes containing surface-exposed BM95 antigenic peptides for the control of cattle tick infestations

Abstract The Rhipicephalus (Boophilus) microplus BM86 and BM95 glycoproteins are homologous proteins that protect cattle against tick infestations. In this study, we demonstrated that the recombinant chimeric protein comprising tick BM95 immunogenic peptides fused to the A. marginale MSP1a N-termina...

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Bibliographic Details
Published in:Vaccine 2009-12, Vol.27 (52), p.7244-7248
Main Authors: Canales, Mario, Labruna, Marcelo B, Soares, João F, Prudencio, Carlos R, de la Fuente, José
Format: Article
Language:English
Subjects:
Allergy and Immunology
Anaplasma
Anaplasma - immunology
Animals
Antibody Formation
Antigens
Applied microbiology
Bacteriology
Biological and medical sciences
Bm86
Bm95
Boophilus
Boophilus microplus
Cattle
Cattle Diseases - immunology
Cattle Diseases - prevention & control
Chimeras
E coli
Escherichia coli - immunology
Experiments
Females
Fermentation
Food contamination & poisoning
Fundamental and applied biological sciences. Psychology
Glycoproteins
Membrane Glycoproteins - immunology
Membranes
Microbiology
Miscellaneous
MSP1a
Peptides
Protein expression
Proteins
Rabbits
Recombinant Fusion Proteins - immunology
Recombinant Proteins - immunology
Rhipicephalus
Tick
Tick Infestations - immunology
Tick Infestations - prevention & control
Tick Infestations - veterinary
Vaccine
Vaccines
Vaccines - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Abstract The Rhipicephalus (Boophilus) microplus BM86 and BM95 glycoproteins are homologous proteins that protect cattle against tick infestations. In this study, we demonstrated that the recombinant chimeric protein comprising tick BM95 immunogenic peptides fused to the A. marginale MSP1a N-terminal region for presentation on the Escherichia coli membrane was protective against R. microplus infestations in rabbits. This system provides a novel and simple approach for the production of tick protective antigens by surface display of antigenic protein chimera on live E. coli and suggests the possibility of using recombinant bacterial membrane fractions for vaccination against cattle tick infestations.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2009.09.123