Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611

Different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611. Moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as al...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2006-12, Vol.33 (12), p.1003-1009
Main Authors: Dorta, C, Cruz, R, Oliva-Neto, P. de, Moura, D.J.C
Format: Article
Language:English
Subjects:
Aspergillus - growth & development
Aspergillus - metabolism
Aspergillus japonicus
Aspergillus niger
Aspergillus niger - growth & development
Aspergillus niger - metabolism
Biological and medical sciences
Biotechnology
cane syrup
Chromatography, High Pressure Liquid
Cultivation
culture media
Culture Media - chemistry
Enzymes
fructooligosaccharides
Fructose - analysis
Fructose - biosynthesis
Fundamental and applied biological sciences. Psychology
Hexosyltransferases - analysis
Hexosyltransferases - biosynthesis
Industrial Microbiology - methods
Liquid chromatography
Molasses
Oligosaccharides - analysis
Oligosaccharides - biosynthesis
Peptones
Peptones - analysis
Powders
Saccharum - chemistry
Saccharum officinarum
Sucrose
Sucrose - analysis
Sugar
Sugarcane
Syrups & sweeteners
Yeast
Yeasts
Yeasts - chemistry
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Summary:Different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611. Moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as alternative nutrients for both strains' cultivation. These media were formulated for analysis of cellular growth, β-Fructosyltransferase and Fructooligosaccharides (FOS) production. Transfructosylating activity (U(t)) and FOS production were analyzed by HPLC. The highest enzyme production by both the strains was 3% (w/v) sucrose and 3% (w/v) peptone, or 3.5% (w/v) total sugars present in cane molasses and 1.5% (w/v) yeast powder. Cane molasses and yeast powder were as good as sucrose and peptone in the enzyme and FOS (around 60% w/w) production by studied strains.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-006-0152-x