Construction of proteinase A deficient transformant of industrial brewing yeast

Yeast proteinase A is detrimental to beer foam. The proteinase A deficient transformant of industrial brewing yeast, WZ65/a, was constructed using PCR-mediated gene disruption, and the transformant was verified to be genetically stable. The PCR analysis showed that PEP4 gene coding for proteinase A...

Full description

Saved in:
Bibliographic Details
Published in:European food research & technology 2007-09, Vol.225 (5-6), p.831-835
Main Authors: WANG, Zhao-Yue, HE, Guo-Qing, HUI RUAN, LIU, Zhong-Shan, YANG, Lu-Fang, ZHANG, Bo-Run
Format: Article
Language:English
Subjects:
Bacteria
Beers
Biological and medical sciences
Construction
Fermented food industries
Food industries
Fundamental and applied biological sciences. Psychology
Microbiology
Yeast
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Yeast proteinase A is detrimental to beer foam. The proteinase A deficient transformant of industrial brewing yeast, WZ65/a, was constructed using PCR-mediated gene disruption, and the transformant was verified to be genetically stable. The PCR analysis showed that PEP4 gene coding for proteinase A in the WZ65/a was disrupted. No matter in the yeast cells or in the fermenting liquor of WZ65/a, proteinase A activity could not be detected. Analysis of the main charicteristics indexes of beer also showed that proteinase A activity and foam performance in the beer brewed with WZ65/a were better than that of the host strain, WZ65.[PUBLICATION ABSTRACT]
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-006-0488-5