Nutrient regulation of epothilone biosynthesis in heterologous and native production strains

Fermentation media with different initial concentrations of ammonium and phosphate salts were used to study the inhibitory effects of those ions on growth and production of epothilone in Sorangium cellulosum and Myxococcus xanthus. The native epothilone producer, S. cellulosum was more sensitive to...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2003-06, Vol.61 (5-6), p.451-455
Main Authors: REGENTIN, R, FRYKMAN, S, LAU, J, TSURUTA, H, LICARI, P
Format: Article
Language:English
Subjects:
Ammonium
Antineoplastic Agents - metabolism
Biological and medical sciences
Biosynthesis
Biotechnology
Cell growth
Culture Media
Epothilones - biosynthesis
Fermentation
Fundamental and applied biological sciences. Psychology
Genetic engineering
Glycerol
Iron - metabolism
Iron - pharmacology
Myxococcales - drug effects
Myxococcales - growth & development
Myxococcales - metabolism
Myxococcus xanthus
Myxococcus xanthus - drug effects
Myxococcus xanthus - growth & development
Myxococcus xanthus - metabolism
Nutrients
Phosphate industry
Phosphates
Phosphates - metabolism
Phosphates - pharmacology
Seeds
Sorangium cellulosum
Studies
Yeast
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Summary:Fermentation media with different initial concentrations of ammonium and phosphate salts were used to study the inhibitory effects of those ions on growth and production of epothilone in Sorangium cellulosum and Myxococcus xanthus. The native epothilone producer, S. cellulosum was more sensitive to ammonium and phosphate than the heterologous producer, M. xanthus. An ammonium concentration of 12 mM reduced epothilone titers by 90% in S. cellulosum but by only 40% in M. xanthus. When 5 mM phosphate was added to the medium, production in both strains was 60% lower. Higher phosphate concentrations had little additional effect on M. xanthus titers, but epothilone production with 17 mM extra-cellular phosphate in S. cellulosum was 95% lower than in the control condition. The effect of iron supplementation to the fermentation medium was also investigated. Both strains showed best production with 20 microM iron added to the medium.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-003-1263-1