Culture conditions improving the production of jadomycin B

Abstract The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection. We have modified the culture conditions by altering the carbon source, buffer, inoculum size, and timing of ethanol sho...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2006-09, Vol.33 (9), p.767-772
Main Authors: Jakeman, David L, Graham, Cathy L, Young, Wendy, Vining, Leo C
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - metabolism
Antibiotics
Bacteria
Bacteriological Techniques - methods
Biological and medical sciences
Biotechnology
Biotechnology - methods
Carbon sources
Chondroitin Sulfate Proteoglycans - metabolism
Culture Media - chemistry
Ethanol
Fundamental and applied biological sciences. Psychology
Glucose - chemistry
Glucose - metabolism
Isoquinolines - chemistry
Isoquinolines - metabolism
Microbiology
Molecular Structure
Morpholines - chemistry
Morpholines - metabolism
Naphthoquinones - chemistry
Naphthoquinones - metabolism
Receptor-Like Protein Tyrosine Phosphatases, Class 5
Streptomyces - classification
Streptomyces - metabolism
Streptomyces venezuelae
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Summary:Abstract The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection. We have modified the culture conditions by altering the carbon source, buffer, inoculum size, and timing of ethanol shock, thereby reducing growing times and improving jadomycin B production. Our optimized conditions use glucose as the carbon source, MOPS as buffer, low concentrations of phosphate, a defined inoculum concentration and an immediate ethanol shock to induce jadomycin B production; results that contrast previous studies. The altered media will facilitate the isolation of related jadomycin B congeners.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-006-0113-4