Development of [3H]muscimol binding to subcellular particles of a culture of mouse brain

"Specific" binding of [3H]muscimol (11.5 nM) related to GABA receptors, occurred in subcellular particles prepared from 4-, 8-, and 12-day cultures of embryonic (14- to 15-day-old) mouse cerebrum, but not to particles prepared from 20- to 45-day cultures. Phase-contrast microscopy revealed...

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Bibliographic Details
Published in:Neurochemical research 1980-12, Vol.5 (12), p.1279-1290
Main Authors: Sarliève, L L, DeFeudis, F V, Ossola, L, Varga, V, Mandel, P
Format: Article
Language:English
Subjects:
Animals
Axons - metabolism
Brain - cytology
Brain - embryology
Brain - metabolism
Cells, Cultured
Female
Mice
Microscopy, Phase-Contrast
Muscimol - metabolism
Neurons - metabolism
Oxazoles - metabolism
Pregnancy
Receptors, Cell Surface - metabolism
Receptors, GABA-A
Subcellular Fractions - metabolism
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Summary:"Specific" binding of [3H]muscimol (11.5 nM) related to GABA receptors, occurred in subcellular particles prepared from 4-, 8-, and 12-day cultures of embryonic (14- to 15-day-old) mouse cerebrum, but not to particles prepared from 20- to 45-day cultures. Phase-contrast microscopy revealed that the presence of neuronal elements (neuroblasts, neurites) paralleled the binding of [3H]muscimol. Further evidence is thus provided for using high-affinity [3H]muscimol binding as a "neuronal marker."
ISSN:0364-3190
1573-6903
DOI:10.1007/BF00964963