Transcription Factor Loading on the MMTV Promoter: A Bimodal Mechanism for Promoter Activation

The mouse mammary tumor virus (MMTV) promoter attains a phased array of six nucleosomes when introduced into rodent cells. This architecture excludes nuclear factor 1/CCAAT transcription factor (NF1/CTF) from the promoter before glucocorticoid treatment and hormone-dependent access of nucleolytic ag...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1992-03, Vol.255 (5051), p.1573-1576
Main Authors: Archer, Trevor K., Lefebvre, Philippe, Wolford, Ronald G., Hager, Gordon L.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding sites
Biochemistry
Biological and medical sciences
CCAAT-Enhancer-Binding Proteins
Cell lines
Cellular biology
Chromatin
Dexamethasone - pharmacology
DNA
DNA probes
DNA, Viral - physiology
DNA-Binding Proteins - physiology
Enzymes
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Viral
Genetic aspects
Genetic regulation
Genetic transcription
Glucocorticoids - physiology
Hormones
In Vitro Techniques
Logical Thinking
Mammary Tumor Virus, Mouse - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mouse mammary tumor virus
NFI Transcription Factors
Nuclear Proteins
Nucleosomes
Nucleosomes - physiology
Oncogenic viruses
Plasmids
Polymorphism, Restriction Fragment Length
Promoter Regions, Genetic - physiology
Promoters (Genetics)
Regulation
Restriction Mapping
Rodentia - genetics
Rodents
Transcription (Genetics)
Transcription factors
Transcription Factors - physiology
Transcription. Transcription factor. Splicing. Rna processing
Transfection
Viruses
Y-Box-Binding Protein 1
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Description
Summary:The mouse mammary tumor virus (MMTV) promoter attains a phased array of six nucleosomes when introduced into rodent cells. This architecture excludes nuclear factor 1/CCAAT transcription factor (NF1/CTF) from the promoter before glucocorticoid treatment and hormone-dependent access of nucleolytic agents to promoter DNA. In contrast, when the promoter was transiently introduced into cells, NF1/CTF was bound constitutively and nucleolytic attack was hormone-independent. Thus, induction at this promoter was a bimodal process involving receptor-dependent remodeling of chromatin that allows NF1/CTF loading and direct receptor-mediated recruitment of additional transcription factors.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1347958