Relationship between Suppression of E6 and E7 Virus Oncogenes and Expression of Apoptosis and Cell Cycle Genes in Cervical Cancer Culture

The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription P...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2010-07, Vol.149 (1), p.73-77
Main Authors: Khokhlova, E. V, Shkoporov, A. N, Volodin, N. N, Efimov, B. A, Pavlov, K. A, Kafarskaia, L. I
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - drug effects
Apoptosis - genetics
Biomedical and Life Sciences
Biomedicine
Cancer
cdc25 Phosphatases - metabolism
Cell Biology
Cell cycle
Cell Cycle Proteins - metabolism
Cervical cancer
Cyclin-Dependent Kinase Inhibitor p21 - metabolism
DNA
DNA microarrays
DNA Primers - genetics
DNA-Binding Proteins - metabolism
Gene Expression Profiling
Gene Expression Regulation, Viral - drug effects
Genes
Genes, cdc - drug effects
Genetic aspects
Genetic transcription
GRB2 Adaptor Protein - metabolism
HeLa Cells
human papilloma viruses
Humans
Infection
Internal Medicine
Laboratory Medicine
Microarray Analysis
Microtubule-Associated Proteins - metabolism
Oncogene Proteins, Viral - metabolism
Oncology
Papillomavirus infections
Pathology
Polo-Like Kinase 1
Protein Serine-Threonine Kinases - metabolism
Proto-Oncogene Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA interference
RNA, Small Interfering - pharmacology
small interfering RNA
viral Oncogenes
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Summary:The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription PCR. Cell transfection with anti-E6 and anti-E7 short interfering RNA moderately reduced the expression of mRNA for CDC25C, GRB2, GTSE1, and PLK1 genes and induced expression of CDKN1A (p21CIP) gene mRNA. In addition, culture proliferation was inhibited and morphological changes characteristic of differentiation and cell aging developed.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-010-0879-2