Carry-over effect of Thidiazuron on banana in vitro proliferation at different culture cycles and light incubation conditions

Thidiazuron (TDZ) is an active cytokinin that was shown to induce increased shoot proliferation and habituation in black walnut, Phaseolus lunatus and evergreen azalea, which are tree species but has not been widely investigated in bananas. Unlike other cytokines commonly in use that are adenine-bas...

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Bibliographic Details
Published in:African journal of biotechnology 2010-05, Vol.9 (21), p.3079-3085
Main Authors: Makara, A M, Rubaihayo, PR, Magambo, MJS
Format: Article
Language:English
Subjects:
Juglans
Musa
Phaseolus lunatus
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Summary:Thidiazuron (TDZ) is an active cytokinin that was shown to induce increased shoot proliferation and habituation in black walnut, Phaseolus lunatus and evergreen azalea, which are tree species but has not been widely investigated in bananas. Unlike other cytokines commonly in use that are adenine-based, TDZ is a urea based cytokinin and therefore is non-degradable by cytokinin-oxidase enzymes in plant tissues. This quality causes TDZ to be persistent in tissues hence transforming them from cytokinin dependence to cytokinin autonomy. This therefore makes use of TDZ cost effective but there is lack of information on this quality in banana micropropagation. A study was therefore conducted to investigate the carry over effect of varying concentrations of TDZ and 22.2 kM benzylaminopurine (BAP) as control on proliferation of five banana cultivars on a hormone free medium under various incubation conditions. The results showed that TDZ had a carry-over effect that enabled shoots to continue proliferating on a hormone free medium as the culture cycles increased and that this effect was significantly (P
ISSN:1684-5315
1684-5315