Selective isolation of Bacillus thuringiensis from soil by use of L-serine as minimal medium supplement

The influence of L-serine on the growth of different strains of the genus Bacillus was investigated. It has been observed that the addition of L-serine to minimal synthetic media results in an inhibition in the growth of certain strains of Bacillus spp. but not B. thuringiensis. Then L-serine-resist...

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Bibliographic Details
Published in:Polish journal of microbiology 2008, Vol.57 (4), p.333-335
Main Authors: Andrzejczak, Sylwia, Lonc, Elzbieta
Format: Article
Language:English
Subjects:
Bacillus
Bacillus thuringiensis
Bacillus thuringiensis - growth & development
Bacillus thuringiensis - isolation & purification
Bacteriological Techniques - methods
Culture Media
Serine
Soil Microbiology
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Summary:The influence of L-serine on the growth of different strains of the genus Bacillus was investigated. It has been observed that the addition of L-serine to minimal synthetic media results in an inhibition in the growth of certain strains of Bacillus spp. but not B. thuringiensis. Then L-serine-resistance phenomenon was used in isolation of B. thuringiensis strains from soil. An isolation method with media supplied with L-serine was compared to the previously applied procedure (isolation on nutrient agar). L-serine-selective medium appeared to be more effective in isolation of Bt strains.
ISSN:1733-1331