A human TERT C-terminal polypeptide sensitizes HeLa cells to H2O2-induced senescence without affecting telomerase enzymatic activity

We have constructed a 27-kDa hTERT C-terminal polypeptide (hTERTC27) devoid of domains required for telomerase activity and demonstrated that it is capable of nuclear translocation/telomere-end targeting. Here we showed that expression of a low level of hTERTC27 renders hTERT positive HeLa cells sen...

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Published in:Biochemical and biophysical research communications 2003-02, Vol.301 (3), p.627-632
Main Authors: Huang, JunJian, Bai, Yun Xiu, Han, Su Wen, Ng, Samuel S M, Jing, Da Dao, Wong, Benjamin C Y, Huang, Cui-fen, Kung, Hsiang-fu, Lin, Marie C M
Format: Article
Language:English
Subjects:
Cellular Senescence
Clone Cells
Cyclin-Dependent Kinase Inhibitor p21
Cyclins - biosynthesis
DNA-Binding Proteins
HeLa Cells
Humans
Hydrogen Peroxide - pharmacology
Oxidants - pharmacology
Oxidative Stress
Peptides - genetics
Peptides - pharmacology
Recombinant Fusion Proteins - pharmacology
Telomerase - chemistry
Telomerase - genetics
Telomerase - metabolism
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Summary:We have constructed a 27-kDa hTERT C-terminal polypeptide (hTERTC27) devoid of domains required for telomerase activity and demonstrated that it is capable of nuclear translocation/telomere-end targeting. Here we showed that expression of a low level of hTERTC27 renders hTERT positive HeLa cells sensitive to H(2)O(2)-induced oxidative stress and subsequent cell senescence. The senescence-associated gene, the cyclin/cdk inhibitor p21(Waf1), was up-regulated. This occurs without changing the expression of endogenous hTERT, causing significant telomere shortening or inhibiting telomerase activity. Results from this study suggest for the first time that in addition to telomerase activity, the C-terminus of hTERT also plays a role in hTERT-mediated cellular resistance to oxidative stress.
ISSN:0006-291X
DOI:10.1016/s0006-291x(02)03049-8