The earthworm Expressed Sequence Tag project

This paper aims to provide a brief description of the earthworm Expressed Sequence Tag (EST) project. ESTs are short single read sequences randomly derived from cDNA libraries and provide the means to acquire large scale sequence information of coding DNA. The earthworm EST project is growing rapidl...

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Bibliographic Details
Published in:Pedobiologia 2003-01, Vol.47 (5), p.447-451
Main Authors: Stürzenbaum, Stephen R., Parkinson, John, Blaxter, Mark, Morgan, A.John, Kille, Peter, Georgiev, Oleg
Format: Article
Language:English
Subjects:
earthworm
EST
Expressed Sequence Tag
sequencing project
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Summary:This paper aims to provide a brief description of the earthworm Expressed Sequence Tag (EST) project. ESTs are short single read sequences randomly derived from cDNA libraries and provide the means to acquire large scale sequence information of coding DNA. The earthworm EST project is growing rapidly and the analysis of the first 577 sequences corresponded to ∼ 400 different genes, with 79 represented by two or more ESTs. Significant sequence similarity to known proteins was observed in 76% of cases and the remaining 24% were classified as novel genes. Using a combination of bioinformatic tools the sequence information was used to build a relational database, LumbriBASE, which can be queried via an internet interface by sequence similarity and key word searches (see http://www.earthworms.org).
ISSN:0031-4056
1873-1511
DOI:10.1078/0031-4056-00211