Nanotopographical stimulation of mechanotransduction and changes in interphase centromere positioning

We apply a recently developed method for controlling the spreading of cultured cells using electron beam lithography (EBL) to create polymethylmethacrylate (PMMA) substrata with repeating nanostructures. There are indications that the reduced cell spreading on these substrata, compared with planar P...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2007-02, Vol.100 (2), p.326-338
Main Authors: Dalby, Matthew J., Biggs, Manus J.P., Gadegaard, Nikolaj, Kalna, Gabriela, Wilkinson, Chris D.W., Curtis, Adam S.G.
Format: Article
Language:English
Subjects:
Cell Adhesion
Cell Line
Cell Nucleus - metabolism
Cell Shape
Centromere - metabolism
Centromere - ultrastructure
cytoskeleton
Cytoskeleton - metabolism
Gene Expression Profiling
Gene Expression Regulation
Humans
Interphase
interphase nucleus organization
mechanotransduction
Mechanotransduction, Cellular
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
nanobioscience
Nanostructures - ultrastructure
nanotopography
Vinculin - metabolism
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Summary:We apply a recently developed method for controlling the spreading of cultured cells using electron beam lithography (EBL) to create polymethylmethacrylate (PMMA) substrata with repeating nanostructures. There are indications that the reduced cell spreading on these substrata, compared with planar PMMA, results from a reduced adhesivity since there are fewer adhesive structures and fewer of their associated stress fibres. The reduced cell spreading also results in a reduced nuclear area and a closer spacing of centrosomes within the nucleus, suggesting that the tension applied to the nucleus is reduced as would be expected from the reduction in stress fibres. In order to obtain further evidence for this, we have used specific inhibitors of components of the cytoskeleton and have found effects comparable with those induced by the new substrata. We have also obtained evidence that these subtrata result in downregulation of gene expression which suggests that this may be due to the changed tension on the nucleus: an intriguing possibility that merits further investigation. J. Cell. Biochem. 100: 326–338, 2007. © 2006 Wiley‐Liss, Inc.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.21058