Interaction of Viologens with Nucleic Acid G-Tetrades

The interaction between the tetrade-forming oligonucleotide 5′-d(T4G4T4) and monoalkylated bipyridinium salts, such as 1-ethyl-4-pyridin-4-ylpyridinium bromide, is reported. The oligomer forms tetrades in the presence of K+ ions but not with Li+. Additionally, the interaction of the thrombin-binding...

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Bibliographic Details
Published in:Langmuir 2008-06, Vol.24 (11), p.5641-5643
Main Authors: Asaftei, Simona, Rosemeyer, Helmut, Walder, Lorenz
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide - chemistry
Chemistry
Colloidal state and disperse state
Electrochemistry
Electrochemistry - methods
Exact sciences and technology
General and physical chemistry
Lithium - chemistry
Oligodeoxyribonucleotides - chemistry
Potassium - chemistry
Surface physical chemistry
Thrombin - chemistry
Viologens - chemistry
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Summary:The interaction between the tetrade-forming oligonucleotide 5′-d(T4G4T4) and monoalkylated bipyridinium salts, such as 1-ethyl-4-pyridin-4-ylpyridinium bromide, is reported. The oligomer forms tetrades in the presence of K+ ions but not with Li+. Additionally, the interaction of the thrombin-binding aptamer 5′-d(GGTTGGTGTGGTTGG) (TBA) with a dialkylated bipyridinium salt, viologen, was studied by cyclic voltammetry. This was performed either on a TiO2 electrode, derivatized with 3-aminopropyltriethoxysilane (APS), using [Fe(CN)6]4− as a marker ion or without a marker ion on an electrostatically TiO2-bound amino-ferrocenyl derivative. Both experiments proof a strong interaction between the immobilized aptamer and the viologen. Third, the electrochemical response of the specific thrombin binding to the immobilized aptamer was studied.
ISSN:0743-7463
1520-5827
DOI:10.1021/la704001n