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Lipid-Mediated Light Activation of a Mechanosensitive Channel of Large Conductance
This paper describes the reversible activation of a mechanosensitive channel via a light-sensitive lipid mimic. For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol...
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Published in: | Langmuir 2004-08, Vol.20 (17), p.6985-6987 |
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creator | Folgering, Joost H. A Kuiper, Johanna M de Vries, Alex H Engberts, Jan B. F. N Poolman, Bert |
description | This paper describes the reversible activation of a mechanosensitive channel via a light-sensitive lipid mimic. For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol % 1,2-dioleoyl-sn-glycero-3-phosphocholine and 20 mol % di-(5-{[4-(4-butylphenyl)azo]phenoxy}pentyl)phosphate (4-Azo-5P). Light-induced isomerization of the azobenzene moiety of 4-Azo-5P from trans to cis was used to activate MscL. |
doi_str_mv | 10.1021/la048942v |
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subjects | Azo Compounds - chemistry Azo Compounds - radiation effects Bacterial Proteins - chemistry Bacterial Proteins - physiology Bacterial Proteins - radiation effects Escherichia coli Escherichia coli - chemistry Escherichia coli - physiology Ion Channels - chemistry Ion Channels - physiology Ion Channels - radiation effects Lactococcus lactis Lactococcus lactis - chemistry Lactococcus lactis - physiology Light Lipid Bilayers - chemistry Lipid Bilayers - radiation effects Lipids - chemistry Lipids - radiation effects Molecular Structure Organophosphorus Compounds - chemistry Organophosphorus Compounds - radiation effects Phosphatidylcholines - chemistry Phosphatidylcholines - radiation effects Stereoisomerism Surface Properties Time Factors |
title | Lipid-Mediated Light Activation of a Mechanosensitive Channel of Large Conductance |
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