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Lipid-Mediated Light Activation of a Mechanosensitive Channel of Large Conductance

This paper describes the reversible activation of a mechanosensitive channel via a light-sensitive lipid mimic. For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol...

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Published in:Langmuir 2004-08, Vol.20 (17), p.6985-6987
Main Authors: Folgering, Joost H. A, Kuiper, Johanna M, de Vries, Alex H, Engberts, Jan B. F. N, Poolman, Bert
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description This paper describes the reversible activation of a mechanosensitive channel via a light-sensitive lipid mimic. For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol % 1,2-dioleoyl-sn-glycero-3-phosphocholine and 20 mol % di-(5-{[4-(4-butylphenyl)azo]phenoxy}pentyl)phosphate (4-Azo-5P). Light-induced isomerization of the azobenzene moiety of 4-Azo-5P from trans to cis was used to activate MscL.
doi_str_mv 10.1021/la048942v
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subjects Azo Compounds - chemistry
Azo Compounds - radiation effects
Bacterial Proteins - chemistry
Bacterial Proteins - physiology
Bacterial Proteins - radiation effects
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - physiology
Ion Channels - chemistry
Ion Channels - physiology
Ion Channels - radiation effects
Lactococcus lactis
Lactococcus lactis - chemistry
Lactococcus lactis - physiology
Light
Lipid Bilayers - chemistry
Lipid Bilayers - radiation effects
Lipids - chemistry
Lipids - radiation effects
Molecular Structure
Organophosphorus Compounds - chemistry
Organophosphorus Compounds - radiation effects
Phosphatidylcholines - chemistry
Phosphatidylcholines - radiation effects
Stereoisomerism
Surface Properties
Time Factors
title Lipid-Mediated Light Activation of a Mechanosensitive Channel of Large Conductance
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