See what you eat—broad GMO screening with microarrays

Despite the controversy of whether genetically modified organisms (GMOs) are beneficial or harmful for humans, animals, and/or ecosystems, the number of cultivated GMOs is increasing every year. Many countries and federations have implemented safety and surveillance systems for GMOs. Potent testing...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2010-03, Vol.396 (6), p.1961-1967
Main Author: von Gotz, Franz
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Biochemistry
Biotechnology
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Crops, Agricultural - genetics
DNA microarrays
Food
Food Science
Forecasts and trends
Gene amplification
Genetic Techniques
Genetically engineered foods
Genetically modified crops
Identification and classification
Labeling
Laboratory Medicine
Laws, regulations and rules
Mathematical analysis
Methods
Monitoring/Environmental Analysis
Nucleotide sequence
Oligonucleotide Array Sequence Analysis
Physiological aspects
Plants, Genetically Modified - genetics
Polymerase chain reaction
Primers (Molecular genetics)
Promoters (Genetics)
Properties
Screening
Trends
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Summary:Despite the controversy of whether genetically modified organisms (GMOs) are beneficial or harmful for humans, animals, and/or ecosystems, the number of cultivated GMOs is increasing every year. Many countries and federations have implemented safety and surveillance systems for GMOs. Potent testing technologies need to be developed and implemented to monitor the increasing number of GMOs. First, these GMO tests need to be comprehensive, i.e., should detect all, or at least the most important, GMOs on the market. This type of GMO screening requires a high degree of parallel tests or multiplexing. To date, DNA microarrays have the highest number of multiplexing capabilities when nucleic acids are analyzed. This trend article focuses on the evolution of DNA microarrays for GMO testing. Over the last 7 years, combinations of multiplex PCR detection and microarray detection have been developed to qualitatively assess the presence of GMOs. One example is the commercially available DualChip® GMO (Eppendorf, Germany; http://www.eppendorf-biochip.com), which is the only GMO screening system successfully validated in a multicenter study. With use of innovative amplification techniques, promising steps have recently been taken to make GMO detection with microarrays quantitative. [graphic removed]
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-009-3204-z