Detection of apolipoprotein A-I, B, and E immunoreactivity in the nuclei of various rat tissue cells

Proteins with apolipoprotein A-I immunoreactivity were detected in the fraction of non-histone acidic proteins isolated from nuclei of various rat tissue cells. These proteins were detected in the brain, liver, kidney, lung, heart, skeletal muscle, testis, spleen, and bone marrow. In the same fracti...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2000-12, Vol.65 (12), p.1419-1423
Main Authors: Panin, L E, Russkikh, G S, Polyakov, L M
Format: Article
Language:English
Subjects:
Animal tissues
Animals
Apolipoprotein A-I - biosynthesis
Apolipoproteins
Apolipoproteins B - biosynthesis
Apolipoproteins E - biosynthesis
Bone marrow
Cell Nucleus - metabolism
Chromatin - metabolism
Immunoblotting
Liver - metabolism
Proteins
Rats
Rats, Wistar
Tissue Distribution
Transcription, Genetic
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Summary:Proteins with apolipoprotein A-I immunoreactivity were detected in the fraction of non-histone acidic proteins isolated from nuclei of various rat tissue cells. These proteins were detected in the brain, liver, kidney, lung, heart, skeletal muscle, testis, spleen, and bone marrow. In the same fraction from liver nuclei, proteins with apoB and apoE immunoreactivity were also detected. The composition of these proteins was studied by immunoblotting. ApoA-I immunoreactivity in the liver nuclei is due to two proteins. One 28-kD protein corresponds to the mature form of the plasma pool of apoA-I and another 14-kD protein is the product of limited proteolysis of apoA-I. The highest content of apoA-I immunoreactivity was detected in transcriptionally active chromatin and nuclear matrix. ApoB immunoreactivity is due to six proteins with molecular weights from 15 to 100 kD. ApoE immunoreactivity is due to a single protein corresponding to the 35-kD form of plasma apoE. Proteins with apoA-I, apoB, and apoE immunoreactivity may be involved in the regulation of transcriptional activity of chromatin.
ISSN:0006-2979
1608-3040
DOI:10.1023/A:1002861008363