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A novel biopesticide production: attagel-mediated precipitation of chitinase from Beauveria bassiana SFB-205 supernatant for thermotolerance
Insect killing fungi have high potential in controlling agriculturally harmful pest, but their slow progress and high variation in killing insect are major impediments to successful industrialization. The present work describes the use of supernatant from the liquid culture of Beauveria bassiana SFB...
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| Published in: | Applied microbiology and biotechnology 2010-08, Vol.87 (5), p.1639-1648 |
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| cites | cdi_FETCH-LOGICAL-c465t-2c5b25a3988e78eb97a76c1108081b49b5f6435ea048931d7960348cb07d32bd3 |
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| container_title | Applied microbiology and biotechnology |
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| creator | Kim, Jae Su Je, Yeon Ho |
| description | Insect killing fungi have high potential in controlling agriculturally harmful pest, but their slow progress and high variation in killing insect are major impediments to successful industrialization. The present work describes the use of supernatant from the liquid culture of
Beauveria bassiana
SFB-205 to surmount this problem, particularly efficient production of thermotolerant chitinase, which is one of the major pathogenesis-related enzymes in the supernatant. The chitinase was precipitated using varying mineral precipitants and followed by lyophilization, which was compared with a salting out method using ammonium sulfate in effectiveness. Incorporating of the supernatant fraction of the
Beauveria
preparation with attagel at 0.5% (
w/v
) as a precipitant enabled this treatment to show the greatest chitinase-precipitation efficiency (93.4%), followed up with excellent insecticidal activity against cotton aphids when it was mixed with 0.01% (
v/v
) polyoxyethylene-(3)-isotridecyl ether (TDE-3) as a spreading agent in laboratory conditions. Consequently, lyophilized attagel-mediated precipitation pellet was superior to lyophilized salting out pellet in maintaining chitinase activity against a thermal stress at 50°C. This finding provides that the attagel-mediated precipitation can be exploited to improve the thermotolerance of
B. bassiana
SFB-205 chitinase as a novel strategy for biopesticide production. |
| doi_str_mv | 10.1007/s00253-010-2543-1 |
| format | article |
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Beauveria bassiana
SFB-205 to surmount this problem, particularly efficient production of thermotolerant chitinase, which is one of the major pathogenesis-related enzymes in the supernatant. The chitinase was precipitated using varying mineral precipitants and followed by lyophilization, which was compared with a salting out method using ammonium sulfate in effectiveness. Incorporating of the supernatant fraction of the
Beauveria
preparation with attagel at 0.5% (
w/v
) as a precipitant enabled this treatment to show the greatest chitinase-precipitation efficiency (93.4%), followed up with excellent insecticidal activity against cotton aphids when it was mixed with 0.01% (
v/v
) polyoxyethylene-(3)-isotridecyl ether (TDE-3) as a spreading agent in laboratory conditions. Consequently, lyophilized attagel-mediated precipitation pellet was superior to lyophilized salting out pellet in maintaining chitinase activity against a thermal stress at 50°C. This finding provides that the attagel-mediated precipitation can be exploited to improve the thermotolerance of
B. bassiana
SFB-205 chitinase as a novel strategy for biopesticide production.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-010-2543-1</identifier><identifier>PMID: 20445976</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Agricultural biotechnology ; Ammonium ; Animals ; Aphididae ; Aphids - drug effects ; Beauveria ; Beauveria - enzymology ; Beauveria bassiana ; Biological and medical sciences ; Biomedical and Life Sciences ; Biopesticides ; Biotechnological Products and Process Engineering ; Biotechnology ; Biotechnology - methods ; Chemical Fractionation - methods ; Chitinase ; Chitinases - isolation & purification ; Chitinases - pharmacology ; Cotton ; Efficiency ; Enzyme Stability ; Enzymes ; Freeze Drying - methods ; Fundamental and applied biological sciences. Psychology ; Fungi ; Glycerol ; Insects ; Killing ; Laboratories ; Life Sciences ; Metabolites ; Microbial Genetics and Genomics ; Microbiology ; Pathogenesis ; Pellets ; Pesticides ; Pesticides - isolation & purification ; Pesticides - pharmacology ; Precipitation ; Protein Stability ; Proteins ; Salting ; Soil sciences ; Studies ; Survival Analysis ; Temperature ; Thermal stress</subject><ispartof>Applied microbiology and biotechnology, 2010-08, Vol.87 (5), p.1639-1648</ispartof><rights>Springer-Verlag 2010</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-2c5b25a3988e78eb97a76c1108081b49b5f6435ea048931d7960348cb07d32bd3</citedby><cites>FETCH-LOGICAL-c465t-2c5b25a3988e78eb97a76c1108081b49b5f6435ea048931d7960348cb07d32bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/613766288/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/613766288?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,11686,27922,27923,36058,36059,44361,74665</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23049989$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20445976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Jae Su</creatorcontrib><creatorcontrib>Je, Yeon Ho</creatorcontrib><title>A novel biopesticide production: attagel-mediated precipitation of chitinase from Beauveria bassiana SFB-205 supernatant for thermotolerance</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Insect killing fungi have high potential in controlling agriculturally harmful pest, but their slow progress and high variation in killing insect are major impediments to successful industrialization. The present work describes the use of supernatant from the liquid culture of
Beauveria bassiana
SFB-205 to surmount this problem, particularly efficient production of thermotolerant chitinase, which is one of the major pathogenesis-related enzymes in the supernatant. The chitinase was precipitated using varying mineral precipitants and followed by lyophilization, which was compared with a salting out method using ammonium sulfate in effectiveness. Incorporating of the supernatant fraction of the
Beauveria
preparation with attagel at 0.5% (
w/v
) as a precipitant enabled this treatment to show the greatest chitinase-precipitation efficiency (93.4%), followed up with excellent insecticidal activity against cotton aphids when it was mixed with 0.01% (
v/v
) polyoxyethylene-(3)-isotridecyl ether (TDE-3) as a spreading agent in laboratory conditions. Consequently, lyophilized attagel-mediated precipitation pellet was superior to lyophilized salting out pellet in maintaining chitinase activity against a thermal stress at 50°C. This finding provides that the attagel-mediated precipitation can be exploited to improve the thermotolerance of
B. bassiana
SFB-205 chitinase as a novel strategy for biopesticide production.</description><subject>Agricultural biotechnology</subject><subject>Ammonium</subject><subject>Animals</subject><subject>Aphididae</subject><subject>Aphids - drug effects</subject><subject>Beauveria</subject><subject>Beauveria - enzymology</subject><subject>Beauveria bassiana</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biopesticides</subject><subject>Biotechnological Products and Process Engineering</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Chemical Fractionation - methods</subject><subject>Chitinase</subject><subject>Chitinases - isolation & purification</subject><subject>Chitinases - pharmacology</subject><subject>Cotton</subject><subject>Efficiency</subject><subject>Enzyme Stability</subject><subject>Enzymes</subject><subject>Freeze Drying - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Glycerol</subject><subject>Insects</subject><subject>Killing</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Metabolites</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Pathogenesis</subject><subject>Pellets</subject><subject>Pesticides</subject><subject>Pesticides - isolation & purification</subject><subject>Pesticides - pharmacology</subject><subject>Precipitation</subject><subject>Protein Stability</subject><subject>Proteins</subject><subject>Salting</subject><subject>Soil sciences</subject><subject>Studies</subject><subject>Survival Analysis</subject><subject>Temperature</subject><subject>Thermal stress</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNqFkUtrFTEYhoMo9nj0B7iRIIhuRnO_uGuLVaHgQl0Pmcw3bcrMZEwyBf-DP9oM52hBUFdZvE--24PQU0peU0L0m0wIk7whlDRMCt7Qe2hHBWcNUVTcRztCtWy0tOYEPcr5hhDKjFIP0QkjQkir1Q79OMVzvIURdyEukEvwoQe8pNivvoQ4v8WuFHcFYzNBH1yBvobgwxKK23IcB-yvQwmzy4CHFCd8Bm69hRQc7lzOwc0Of744axiROK8LpNkVNxc8xITLNaQpljhCcrOHx-jB4MYMT47vHn29ePfl_ENz-en9x_PTy8YLJUvDvOyYdNwaA9pAZ7XTylNKDDG0E7aTgxJcgiPCWE57bRXhwviO6J6zrud79PJQt-75ba1bt1PIHsbRzRDX3GopjOFS6_-TnFttNaeVfPVPsqrgknAlbUWf_4HexLWeZcytolwrxWr3PaIHyKeYc4KhXVKYXPreUtJu9tuD_bbabzf77TbDs2Phtau6fv_4pbsCL46Ay96Nw3b1kO84ToS1ZpuQHbhco_kK0t2Ef-_-Eyk6xuc</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Kim, Jae Su</creator><creator>Je, Yeon Ho</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7SU</scope><scope>7U5</scope><scope>F28</scope><scope>L7M</scope><scope>7X8</scope><scope>7QH</scope><scope>7QO</scope><scope>7SS</scope><scope>7UA</scope></search><sort><creationdate>20100801</creationdate><title>A novel biopesticide production: attagel-mediated precipitation of chitinase from Beauveria bassiana SFB-205 supernatant for thermotolerance</title><author>Kim, Jae Su ; Je, Yeon Ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-2c5b25a3988e78eb97a76c1108081b49b5f6435ea048931d7960348cb07d32bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Agricultural biotechnology</topic><topic>Ammonium</topic><topic>Animals</topic><topic>Aphididae</topic><topic>Aphids - drug effects</topic><topic>Beauveria</topic><topic>Beauveria - enzymology</topic><topic>Beauveria bassiana</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biopesticides</topic><topic>Biotechnological Products and Process Engineering</topic><topic>Biotechnology</topic><topic>Biotechnology - methods</topic><topic>Chemical Fractionation - methods</topic><topic>Chitinase</topic><topic>Chitinases - isolation & purification</topic><topic>Chitinases - pharmacology</topic><topic>Cotton</topic><topic>Efficiency</topic><topic>Enzyme Stability</topic><topic>Enzymes</topic><topic>Freeze Drying - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>Glycerol</topic><topic>Insects</topic><topic>Killing</topic><topic>Laboratories</topic><topic>Life Sciences</topic><topic>Metabolites</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Pathogenesis</topic><topic>Pellets</topic><topic>Pesticides</topic><topic>Pesticides - isolation & purification</topic><topic>Pesticides - pharmacology</topic><topic>Precipitation</topic><topic>Protein Stability</topic><topic>Proteins</topic><topic>Salting</topic><topic>Soil sciences</topic><topic>Studies</topic><topic>Survival Analysis</topic><topic>Temperature</topic><topic>Thermal stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jae Su</creatorcontrib><creatorcontrib>Je, Yeon Ho</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Business Premium Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Biological Sciences</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Environmental Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Water Resources Abstracts</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jae Su</au><au>Je, Yeon Ho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel biopesticide production: attagel-mediated precipitation of chitinase from Beauveria bassiana SFB-205 supernatant for thermotolerance</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>87</volume><issue>5</issue><spage>1639</spage><epage>1648</epage><pages>1639-1648</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>Insect killing fungi have high potential in controlling agriculturally harmful pest, but their slow progress and high variation in killing insect are major impediments to successful industrialization. The present work describes the use of supernatant from the liquid culture of
Beauveria bassiana
SFB-205 to surmount this problem, particularly efficient production of thermotolerant chitinase, which is one of the major pathogenesis-related enzymes in the supernatant. The chitinase was precipitated using varying mineral precipitants and followed by lyophilization, which was compared with a salting out method using ammonium sulfate in effectiveness. Incorporating of the supernatant fraction of the
Beauveria
preparation with attagel at 0.5% (
w/v
) as a precipitant enabled this treatment to show the greatest chitinase-precipitation efficiency (93.4%), followed up with excellent insecticidal activity against cotton aphids when it was mixed with 0.01% (
v/v
) polyoxyethylene-(3)-isotridecyl ether (TDE-3) as a spreading agent in laboratory conditions. Consequently, lyophilized attagel-mediated precipitation pellet was superior to lyophilized salting out pellet in maintaining chitinase activity against a thermal stress at 50°C. This finding provides that the attagel-mediated precipitation can be exploited to improve the thermotolerance of
B. bassiana
SFB-205 chitinase as a novel strategy for biopesticide production.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>20445976</pmid><doi>10.1007/s00253-010-2543-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2010-08, Vol.87 (5), p.1639-1648 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_754883577 |
| source | ABI/INFORM Global; Springer Nature |
| subjects | Agricultural biotechnology Ammonium Animals Aphididae Aphids - drug effects Beauveria Beauveria - enzymology Beauveria bassiana Biological and medical sciences Biomedical and Life Sciences Biopesticides Biotechnological Products and Process Engineering Biotechnology Biotechnology - methods Chemical Fractionation - methods Chitinase Chitinases - isolation & purification Chitinases - pharmacology Cotton Efficiency Enzyme Stability Enzymes Freeze Drying - methods Fundamental and applied biological sciences. Psychology Fungi Glycerol Insects Killing Laboratories Life Sciences Metabolites Microbial Genetics and Genomics Microbiology Pathogenesis Pellets Pesticides Pesticides - isolation & purification Pesticides - pharmacology Precipitation Protein Stability Proteins Salting Soil sciences Studies Survival Analysis Temperature Thermal stress |
| title | A novel biopesticide production: attagel-mediated precipitation of chitinase from Beauveria bassiana SFB-205 supernatant for thermotolerance |
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