Putative Recombination Events and Evolutionary History of Five Economically Important Viruses of Fruit Trees Based on Coat Protein-Encoding Gene Sequence Analysis

• Published in: Biochemical genetics 2010-06, Vol.48 (5-6), p.357-375
• Main Author: Boulila, Moncef
• Format: Article
• Language: English
• Subjects: Biochemistry; bioinformatics; Biomedical and Life Sciences; Biomedicine; Capsid Proteins - genetics; Coat protein gene; Databases, Genetic; Economic importance; Evolution, Molecular; Fruit; Fruit crops; Fruit trees; Fruit viruses; Fruits; Genes, Viral - genetics; Human Genetics; Medical Microbiology; Phylogeny; Recombination; Recombination, Genetic; Sequence; Sequence Analysis, DNA; Trees - virology; Viruses - genetics; Zoology
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1007/s10528-009-9317-4

To enhance the knowledge of recombination as an evolutionary process, 267 accessions retrieved from GenBank were investigated, all belonging to five economically important viruses infecting fruit crops (Plum pox, Apple chlorotic leaf spot, Apple mosaic, Prune dwarf, and Prunus necrotic ringspot viruses). Putative recombinational events were detected in the coat protein (CP)-encoding gene using RECCO and RDP version 3.31β algorithms. Based on RECCO results, all five viruses were shown to contain potential recombination signals in the CP gene. Reconstructed trees with modified topologies were proposed. Furthermore, RECCO performed better than the RDP package in detecting recombination events and exhibiting their evolution rate along the sequences of the five viruses. RDP, however, provided the possible major and minor parents of the recombinants. Thus, the two methods should be considered complementary.