Molecular Characterization and Expression Pattern of Fetuin-B in Gibel Carp (Carassius auratus gibelio)

Fetuin-B has recently been cloned and identified from rats, mice, and humans; their expression patterns, however, have not been elucidated yet. In this study, Cagfetuin-B has been cloned in gibel carp. RT-PCR and Western blot detection revealed that Cagfetuin-B is first transcribed from the blastula...

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Bibliographic Details
Published in:Biochemical genetics 2008-10, Vol.46 (9-10), p.620-633
Main Authors: Liu, Jing-Xia, Zhai, Yan-Hua, Geng, Fan-Suo, Xia, Jian-Hong, Gui, Jian-Fang
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - biosynthesis
alpha-Fetoproteins - genetics
Amino Acid Sequence
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Carassius auratus gibelio
Carps
Embryonic growth stage
Female
Freshwater
Gene Expression Profiling
Gene Expression Regulation
Human Genetics
Larvae
Liver - metabolism
Medical Microbiology
Models, Genetic
Molecular Sequence Data
Ovary - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Tissue Distribution
Transcription, Genetic
Zoology
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Summary:Fetuin-B has recently been cloned and identified from rats, mice, and humans; their expression patterns, however, have not been elucidated yet. In this study, Cagfetuin-B has been cloned in gibel carp. RT-PCR and Western blot detection revealed that Cagfetuin-B is first transcribed from the blastula stage and at a relatively stable level afterward during embryogenesis and the larval stage. Cagfetuin-B transcripts are predominantly distributed over the yolk syncytial layer in the early embryos and later restricted to the cells of liver and brain in newly hatched larvae. Moreover, a dynamic distribution of Cagfetuin-B protein was observed in brain, kidney, liver, and skin during morphogenesis. In adult fish, Cagfetuin-B transcripts are restricted in liver and ovary. Our work, for the first time, revealed the extra-hepatic transcription and a dynamic distribution of fetuin-B during embryogenesis and in adults, which indicates the potential roles of fetuin-B in fish organogenesis.
ISSN:0006-2928
1573-4927
DOI:10.1007/s10528-008-9176-4