Procaine Enhancement of the Rapid Cooling Contracture and Inhibition of the Decay of Potentiated State in Rabbit Papillary Muscle

We tested the possibility that inhibition of Ca 2+release from the sarcoplasmic reticulum decreases the transsarcolemmal Ca 2+efflux and results in an increased sarcoplasmic reticulum Ca 2+content. We used procaine to inhibit sarcoplasmic reticulum Ca 2+release in rabbit papillary muscles. Rapid coo...

Full description

Saved in:
Bibliographic Details
Published in:Journal of molecular and cellular cardiology 1995-12, Vol.27 (12), p.2543-2550
Main Authors: Komai, Hirochika, Redon, David, Rusy, Ben F.
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Heart Ventricles - drug effects
In Vitro Techniques
Muscle Contraction
Papillary Muscles - drug effects
Potentiated state
Procaine
Procaine - pharmacology
Rabbit papillary muscle
Rabbits
Rapid cooling contracture
Sarcoplasmic reticulum
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We tested the possibility that inhibition of Ca 2+release from the sarcoplasmic reticulum decreases the transsarcolemmal Ca 2+efflux and results in an increased sarcoplasmic reticulum Ca 2+content. We used procaine to inhibit sarcoplasmic reticulum Ca 2+release in rabbit papillary muscles. Rapid cooling-induced contractures were used as an index of sarcoplasmic reticulum Ca 2+content. Decay of potentiated state was measured to study whether the transsarcolemmal Ca 2+efflux is decreased in the presence of procaine. Procaine (0.5, 1.0 m m) inhibited contractions elicited by electrical stimulation and enhanced the rapid cooling-induced contracture. The initial decay of potentiated state that occurs when the muscle was stimulated at a low frequency was slowed in the presence of procaine. These results suggest that procaine inhibition of Ca 2+-induced release of sarcoplasmic reticulum Ca 2+decreases the transsarcolemmal Ca 2+efflux relative to the influx, resulting in an increased sarcoplasmic reticulum Ca 2+content.
ISSN:0022-2828
1095-8584
DOI:10.1006/jmcc.1995.0041