Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation

Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple exponential function of the radiation dose. Classical...

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Bibliographic Details
Published in:The Journal of biological chemistry 1981-02, Vol.256 (3), p.1305-1306
Main Authors: Cuppoletti, J, Jung, C Y, Green, F A
Format: Article
Language:English
Subjects:
Biological Transport, Active
Blood Glucose - metabolism
Carrier Proteins - metabolism
Carrier Proteins - radiation effects
Cell Membrane Permeability
Cytochalasin B - pharmacology
Diffusion
Erythrocyte Membrane - metabolism
Erythrocytes - metabolism
Humans
Isomerism
Kinetics
Methylglucosides - blood
Methylglycosides - blood
Molecular Weight
Monosaccharide Transport Proteins
Monosaccharides - metabolism
Monosaccharides - radiation effects
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Description
Summary:Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple exponential function of the radiation dose. Classical target size analysis of this data yielded a molecular size of 185,000 daltons for the carrier. This represents the first measurement of the functional size of a transport protein based directly on flux inactivation.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)69964-3