Loading…
Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation
Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple exponential function of the radiation dose. Classical...
Saved in:
| Published in: | The Journal of biological chemistry 1981-02, Vol.256 (3), p.1305-1306 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c378t-b89c17f8a1dce9752312d37723897644a5211ba28e86babe32e638846aea5f6d3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c378t-b89c17f8a1dce9752312d37723897644a5211ba28e86babe32e638846aea5f6d3 |
| container_end_page | 1306 |
| container_issue | 3 |
| container_start_page | 1305 |
| container_title | The Journal of biological chemistry |
| container_volume | 256 |
| creator | Cuppoletti, J Jung, C Y Green, F A |
| description | Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained
their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple
exponential function of the radiation dose. Classical target size analysis of this data yielded a molecular size of 185,000
daltons for the carrier. This represents the first measurement of the functional size of a transport protein based directly
on flux inactivation. |
| doi_str_mv | 10.1016/S0021-9258(19)69964-3 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75506203</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>75506203</sourcerecordid><originalsourceid>FETCH-LOGICAL-c378t-b89c17f8a1dce9752312d37723897644a5211ba28e86babe32e638846aea5f6d3</originalsourceid><addsrcrecordid>eNo9kEuLFTEQRoMo43X0JwwEF6KLHlNJ57WUQUdhQPAB7kI6XT0d6e5ck7R6_fXeF1ObWnznq4JDyBWwa2Cg3n5ljENjuTSvwb5R1qq2EY_IBpgRjZDw4zHZPCBPybNSfrL9tBYuyIUGyzXAhsy30xpSQVqzX8o25UqDzzlipmmg4zr7hWLe1TGnsKtYrukX30dfY1po9fkeKy3xH9IZfVkzzrhU2vmCPd0Dw7T-pXHxocbfx8pz8mTwU8EX531Jvn94_-3mY3P3-fbTzbu7JghtatMZG0APxkMf0GrJBfBeaM2FsVq1rZccoPPcoFGd71BwVMKYVnn0clC9uCSvTne3Of1asVQ3xxJwmvyCaS1OS8kUZ2IPyhMYciol4-C2Oc4-7xwwd9DsjprdwaED646a3aF3dX6wdjP2D62z133-8pSP8X78EzO6LqYw4uy4VE44EEyK_5E4heA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75506203</pqid></control><display><type>article</type><title>Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation</title><source>ScienceDirect (Online service)</source><creator>Cuppoletti, J ; Jung, C Y ; Green, F A</creator><creatorcontrib>Cuppoletti, J ; Jung, C Y ; Green, F A</creatorcontrib><description>Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained
their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple
exponential function of the radiation dose. Classical target size analysis of this data yielded a molecular size of 185,000
daltons for the carrier. This represents the first measurement of the functional size of a transport protein based directly
on flux inactivation.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)69964-3</identifier><identifier>PMID: 7192711</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Biological Transport, Active ; Blood Glucose - metabolism ; Carrier Proteins - metabolism ; Carrier Proteins - radiation effects ; Cell Membrane Permeability ; Cytochalasin B - pharmacology ; Diffusion ; Erythrocyte Membrane - metabolism ; Erythrocytes - metabolism ; Humans ; Isomerism ; Kinetics ; Methylglucosides - blood ; Methylglycosides - blood ; Molecular Weight ; Monosaccharide Transport Proteins ; Monosaccharides - metabolism ; Monosaccharides - radiation effects</subject><ispartof>The Journal of biological chemistry, 1981-02, Vol.256 (3), p.1305-1306</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-b89c17f8a1dce9752312d37723897644a5211ba28e86babe32e638846aea5f6d3</citedby><cites>FETCH-LOGICAL-c378t-b89c17f8a1dce9752312d37723897644a5211ba28e86babe32e638846aea5f6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7192711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cuppoletti, J</creatorcontrib><creatorcontrib>Jung, C Y</creatorcontrib><creatorcontrib>Green, F A</creatorcontrib><title>Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained
their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple
exponential function of the radiation dose. Classical target size analysis of this data yielded a molecular size of 185,000
daltons for the carrier. This represents the first measurement of the functional size of a transport protein based directly
on flux inactivation.</description><subject>Biological Transport, Active</subject><subject>Blood Glucose - metabolism</subject><subject>Carrier Proteins - metabolism</subject><subject>Carrier Proteins - radiation effects</subject><subject>Cell Membrane Permeability</subject><subject>Cytochalasin B - pharmacology</subject><subject>Diffusion</subject><subject>Erythrocyte Membrane - metabolism</subject><subject>Erythrocytes - metabolism</subject><subject>Humans</subject><subject>Isomerism</subject><subject>Kinetics</subject><subject>Methylglucosides - blood</subject><subject>Methylglycosides - blood</subject><subject>Molecular Weight</subject><subject>Monosaccharide Transport Proteins</subject><subject>Monosaccharides - metabolism</subject><subject>Monosaccharides - radiation effects</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><recordid>eNo9kEuLFTEQRoMo43X0JwwEF6KLHlNJ57WUQUdhQPAB7kI6XT0d6e5ck7R6_fXeF1ObWnznq4JDyBWwa2Cg3n5ljENjuTSvwb5R1qq2EY_IBpgRjZDw4zHZPCBPybNSfrL9tBYuyIUGyzXAhsy30xpSQVqzX8o25UqDzzlipmmg4zr7hWLe1TGnsKtYrukX30dfY1po9fkeKy3xH9IZfVkzzrhU2vmCPd0Dw7T-pXHxocbfx8pz8mTwU8EX531Jvn94_-3mY3P3-fbTzbu7JghtatMZG0APxkMf0GrJBfBeaM2FsVq1rZccoPPcoFGd71BwVMKYVnn0clC9uCSvTne3Of1asVQ3xxJwmvyCaS1OS8kUZ2IPyhMYciol4-C2Oc4-7xwwd9DsjprdwaED646a3aF3dX6wdjP2D62z133-8pSP8X78EzO6LqYw4uy4VE44EEyK_5E4heA</recordid><startdate>19810210</startdate><enddate>19810210</enddate><creator>Cuppoletti, J</creator><creator>Jung, C Y</creator><creator>Green, F A</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810210</creationdate><title>Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation</title><author>Cuppoletti, J ; Jung, C Y ; Green, F A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-b89c17f8a1dce9752312d37723897644a5211ba28e86babe32e638846aea5f6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Biological Transport, Active</topic><topic>Blood Glucose - metabolism</topic><topic>Carrier Proteins - metabolism</topic><topic>Carrier Proteins - radiation effects</topic><topic>Cell Membrane Permeability</topic><topic>Cytochalasin B - pharmacology</topic><topic>Diffusion</topic><topic>Erythrocyte Membrane - metabolism</topic><topic>Erythrocytes - metabolism</topic><topic>Humans</topic><topic>Isomerism</topic><topic>Kinetics</topic><topic>Methylglucosides - blood</topic><topic>Methylglycosides - blood</topic><topic>Molecular Weight</topic><topic>Monosaccharide Transport Proteins</topic><topic>Monosaccharides - metabolism</topic><topic>Monosaccharides - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cuppoletti, J</creatorcontrib><creatorcontrib>Jung, C Y</creatorcontrib><creatorcontrib>Green, F A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cuppoletti, J</au><au>Jung, C Y</au><au>Green, F A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1981-02-10</date><risdate>1981</risdate><volume>256</volume><issue>3</issue><spage>1305</spage><epage>1306</epage><pages>1305-1306</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained
their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple
exponential function of the radiation dose. Classical target size analysis of this data yielded a molecular size of 185,000
daltons for the carrier. This represents the first measurement of the functional size of a transport protein based directly
on flux inactivation.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>7192711</pmid><doi>10.1016/S0021-9258(19)69964-3</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1981-02, Vol.256 (3), p.1305-1306 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75506203 |
| source | ScienceDirect (Online service) |
| subjects | Biological Transport, Active Blood Glucose - metabolism Carrier Proteins - metabolism Carrier Proteins - radiation effects Cell Membrane Permeability Cytochalasin B - pharmacology Diffusion Erythrocyte Membrane - metabolism Erythrocytes - metabolism Humans Isomerism Kinetics Methylglucosides - blood Methylglycosides - blood Molecular Weight Monosaccharide Transport Proteins Monosaccharides - metabolism Monosaccharides - radiation effects |
| title | Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T07%3A29%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glucose%20transport%20carrier%20of%20human%20erythrocytes.%20Radiation%20target%20size%20measurement%20based%20on%20flux%20inactivation&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Cuppoletti,%20J&rft.date=1981-02-10&rft.volume=256&rft.issue=3&rft.spage=1305&rft.epage=1306&rft.pages=1305-1306&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1016/S0021-9258(19)69964-3&rft_dat=%3Cproquest_cross%3E75506203%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c378t-b89c17f8a1dce9752312d37723897644a5211ba28e86babe32e638846aea5f6d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=75506203&rft_id=info:pmid/7192711&rfr_iscdi=true |